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Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus Subtilis

Overview
Journal Eur J Biochem
Specialty Biochemistry
Date 1976 May 17
PMID 819269
Citations 24
Authors
R P Mellado
E Vinuela
M Salas
Affiliations
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Abstract

By treatment of Bacillus subtilis MO-101-P spoA- met thr- su- with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA- [met-]+thr- su+44, was isolated. This strain does not suppress phage phi 29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor.

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