Factors Involved in the Initiation of Phage Phi 29 DNA Replication in Vitro: Requirement of the Gene 2 Product for the Formation of the Protein P3-dAMP Complex

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Mar 11

PMID 6402761

Citations 11

Authors

L Blanco

J A Garcia

M A Penalva

M Salas

Affiliations

Soon will be listed here.

Abstract

To study the requirements for the in vitro formation of the protein p3-dAMP complex, the first step in phi29 DNA replication, extracts from B. subtilis infected with phi29 mutants in genes 2, 3, 5, 6 and 17, involved in DNA synthesis, have been used. The formation of the initiation complex is completely dependent on the presence of a functional gene 2 product, in addition to protein p3 and phi29 DNA-protein p3 as template. ATP is also required, although it can be replaced by other nucleotides. The products of genes 5, 6 and 17 do not seem to be needed in the formation of the initiation complex. Inhibitors of the host DNA polymerase III, DNA gyrase or RNA polymerase had no effect on the formation of the protein p3-dAMP complex, suggesting that these proteins are not involved in the initiation of phi29 DNA replication. ddATP or aphidicolin, inhibitors of DNA chain elongation, had also no effect on the formation of the initiation complex.

Citing Articles

Bacteriophage phi 29 DNA replication in vitro: participation of the terminal protein and the gene 2 product in elongation.

Matsumoto K, Saito T, Kim C, Ando T, Hirokawa H Mol Gen Genet. 1984; 196(3):381-6.

PMID: 6438445 DOI: 10.1007/BF00436183.

Replication of bacteriophage phi 29 DNA in vitro: the roles of terminal protein and DNA polymerase.

Watabe K, Leusch M, Ito J Proc Natl Acad Sci U S A. 1984; 81(17):5374-8.

PMID: 6433349 PMC: 391706. DOI: 10.1073/pnas.81.17.5374.

Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication.

Blanco L, Salas M Proc Natl Acad Sci U S A. 1984; 81(17):5325-9.

PMID: 6433348 PMC: 391696. DOI: 10.1073/pnas.81.17.5325.

Purification in a functional form of the terminal protein of Bacillus subtilis phage phi 29.

Prieto I, Lazaro J, Garcia J, Hermoso J, Salas M Proc Natl Acad Sci U S A. 1984; 81(6):1639-43.

PMID: 6424120 PMC: 344973. DOI: 10.1073/pnas.81.6.1639.

A novel DNA polymerase induced by Bacillus subtilis phage phi 29.

Watabe K, Ito J Nucleic Acids Res. 1983; 11(23):8333-42.

PMID: 6424095 PMC: 326585. DOI: 10.1093/nar/11.23.8333.

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