Peptide Nucleic Acid.DNA Strand Displacement Loops As Artificial Transcription Promoters

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1994 Apr 26

PMID 8171008

Citations 33

Authors

N E Mollegaard

O Buchardt

M Egholm

P E Nielsen

Affiliations

Soon will be listed here.

Abstract

Homopyrimidine peptide nucleic acids (PNAs) form loop structures when binding to complementary double-stranded DNA by strand displacement, and we now show that RNA polymerase recognizes these and initiates RNA transcription from PNA/double-stranded DNA strand displacement complexes at an efficiency comparable to that of the strong Escherichia coli lacUV5 promoter. Thus PNA targets can be considered as artificial promoters controlled positively by the corresponding PNA as a transcription factor. Our results have implications for the mechanism of action of RNA polymerase and suggest the use of PNA as specific gene activating reagents and drugs.

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