Chromosomal Locations of Four Minor RDNA Loci and a Marker Microsatellite Sequence in Barley

Overview

Journal Chromosome Res

Date 1994 Jan 1

PMID 8162323

Citations 27

Authors

C Pedersen

I Linde-Laursen

Affiliations

Soon will be listed here.

Abstract

Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7l), 2 (2l), 4 (4l), and 5 (1l) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have designated the new locus Nor-l6. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

Citing Articles

Comparative Cytogenetic of the 36-Chromosomes Genera of Subtribe (Orchidaceae) in the Mediterranean Region: A Summary and New Data.

Turco A, Albano A, Medagli P, Wagensommer R, DEmerico S Plants (Basel). 2023; 12(15).

PMID: 37570952 PMC: 10421308. DOI: 10.3390/plants12152798.

Physical localization of 45S rDNA in Cymbopogon and the analysis of differential distribution of rDNA in homologous chromosomes of Cymbopogon winterianus.

Thakur S, Kumar U, Malik R, Bisht D, Balyan P, Mir R PLoS One. 2021; 16(11):e0257115.

PMID: 34793445 PMC: 8601443. DOI: 10.1371/journal.pone.0257115.

Molecular cytogenetic and morphological characterization of two wheat-barley translocation lines.

Ivanizs L, Farkas A, Linc G, Molnar-Lang M, Molnar I PLoS One. 2018; 13(6):e0198758.

PMID: 29889875 PMC: 5995406. DOI: 10.1371/journal.pone.0198758.

Addition of Manas barley chromosome arms to the hexaploid wheat genome.

Turkosi E, Cseh A, Darko E, Molnar-Lang M BMC Genet. 2016; 17(1):87.

PMID: 27328706 PMC: 4915093. DOI: 10.1186/s12863-016-0393-2.

Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences.

Svitashev S, Bryngelsson T, Vershinin A, Pedersen C, Sall T, von Bothmer R Theor Appl Genet. 2013; 89(7-8):801-10.

PMID: 24178086 DOI: 10.1007/BF00224500.

References

Dolezel J, Cihalikova J, Lucretti S . A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L. Planta. 2013; 188(1):93-8. DOI: 10.1007/BF00198944. View

Brown G, Amarasinghe V, Kiss G, Carlson J . Preliminary karyotype and chromosomal localization of ribosomal DNA sites in white spruce using fluorescence in situ hybridization. Genome. 1993; 36(2):310-6. DOI: 10.1139/g93-043. View

Lichter P, Tang C, Call K, Hermanson G, Evans G, Housman D . High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science. 1990; 247(4938):64-9. DOI: 10.1126/science.2294592. View

Gerlach W, Bedbrook J . Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979; 7(7):1869-85. PMC: 342353. DOI: 10.1093/nar/7.7.1869. View

Cherif D, Bernard O, Berger R . Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes. Hum Genet. 1989; 81(4):358-62. DOI: 10.1007/BF00283691. View

Baldini A, ROSS M, Nizetic D, Vatcheva R, Lindsay E, Lehrach H . Chromosomal assignment of human YAC clones by fluorescence in situ hybridization: use of single-yeast-colony PCR and multiple labeling. Genomics. 1992; 14(1):181-4. DOI: 10.1016/s0888-7543(05)80303-9. View

Hagemann S, Scheer B, Schweizer D . Repetitive sequences in the genome of Anemone blanda: identification of tandem arrays and of dispersed repeats. Chromosoma. 1993; 102(5):312-24. DOI: 10.1007/BF00661274. View

Baldini A, Ward D . In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. Genomics. 1991; 9(4):770-4. DOI: 10.1016/0888-7543(91)90374-n. View

Pendas A, Moran P . Multi-chromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chromosome Res. 1993; 1(1):63-7. DOI: 10.1007/BF00710608. View

10.

Cherif D, Julier C, Delattre O, Derre J, Lathrop G, Berger R . Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of human chromosome 11. Proc Natl Acad Sci U S A. 1990; 87(17):6639-43. PMC: 54592. DOI: 10.1073/pnas.87.17.6639. View

11.

Pinkel D, Straume T, Gray J . Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci U S A. 1986; 83(9):2934-8. PMC: 323421. DOI: 10.1073/pnas.83.9.2934. View

12.

Meinkoth J, Wahl G . Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984; 138(2):267-84. DOI: 10.1016/0003-2697(84)90808-x. View

13.

Sardana R, Odell M, Flavell R . Correlation between the size of the intergenic regulatory region, the status of cytosine methylation of rRNA genes and nucleolar expression in wheat. Mol Gen Genet. 1993; 236(2-3):155-62. DOI: 10.1007/BF00277107. View

14.

Lawrence J, Villnave C, Singer R . Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell. 1988; 52(1):51-61. DOI: 10.1016/0092-8674(88)90530-2. View

15.

Trask B, Pinkel D, van den Engh G . The proximity of DNA sequences in interphase cell nuclei is correlated to genomic distance and permits ordering of cosmids spanning 250 kilobase pairs. Genomics. 1989; 5(4):710-7. DOI: 10.1016/0888-7543(89)90112-2. View

16.

Leitch I, Heslop-Harrison J . Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare). Genome. 1993; 36(3):517-23. DOI: 10.1139/g93-071. View

17.

Selleri L, Hermanson G, Eubanks J, Evans G . Chromosomal in situ hybridization using yeast artificial chromosomes. Genet Anal Tech Appl. 1991; 8(2):59-66. DOI: 10.1016/1050-3862(91)90050-2. View

18.

Fan Y, Davis L, Shows T . Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosomes. Proc Natl Acad Sci U S A. 1990; 87(16):6223-7. PMC: 54505. DOI: 10.1073/pnas.87.16.6223. View

19.

Heslop-Harrison J, HARRISON G, Leitch I . Reprobing of DNA:DNA in situ hybridization preparations. Trends Genet. 1992; 8(11):372-3. DOI: 10.1016/0168-9525(92)90287-e. View

20.

Krenik K, Kephart G, Offord K, Dunnette S, Gleich G . Comparison of antifading agents used in immunofluorescence. J Immunol Methods. 1989; 117(1):91-7. DOI: 10.1016/0022-1759(89)90122-1. View