Comparative Genomic in Situ Hybridization (cGISH) Analysis on Plant Chromosomes Revealed by Labelled Arabidopsis DNA

Overview

Journal Chromosome Res

Date 2001 Jul 13

PMID 11448038

Citations 1

Authors

J F Zoller

Y Yang

R G Herrmann

U Hohmann

Affiliations

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Abstract

A new approach for comparative cytogenetic banding analysis of plant chromosomes has been established. The comparative GISH (cGISH) technique is universally applicable to various complex genomes of Monocotyledonae (Triticum aestivum, Agropyron elongatum, Secale cereale, Hordeum vulgare, Allium cepa, Muscari armenaticum and Lilium longiflorum) and Dicotyledonae (Vicia faba, Beta vulgaris, Arabidopsis thaliana). Labelled total genomic DNA of A. thaliana generates signals at conserved chromosome regions. The nucleolus organizing regions (NORs) containing the majority of tandemly repeated rDNA sequences, N-band regions containing satellite DNA, conserved homologous sequences at telomeres and additional chromosome-characteristic markers were detected in heterologous FISH experiments. Multicolour FISH analysis with repetitive DNA probes simultaneously revealed the chromosome assignment of 56 cGISH signals in rye and 61 cGISH signals in barley. Further advantages of this technique are: (1) the fast and straightforward preparation of the probe; (2) the generation of signals with high intensity and reproducibility even without signal amplification; and (3) no requirement of species-specific sequences suitable for molecular karyotype analysis. Hybridization can be performed without competitive DNA. Signal detection without significant background is possible under low stringency conditions. The universal application of this fast and simple one-step fluorescence banding technique for plant cytogenetic and plant genome evolution is discussed.

Citing Articles

Molecular cytogenetic characterization and phylogenetic analysis of four species (Poaceae).

Tang Y, Xiao L, Iqbal Y, Liao J, Xiao L, Yi Z Comp Cytogenet. 2019; 13(3):211-230.

PMID: 31428293 PMC: 6697684. DOI: 10.3897/CompCytogen.v13i3.35346.

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