GDI1 Encodes a GDP Dissociation Inhibitor That Plays an Essential Role in the Yeast Secretory Pathway

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1994 Apr 1

PMID 8157010

Citations 66

Authors

M D Garrett

J E Zahner

C M Cheney

P J Novick

Affiliations

Soon will be listed here.

Abstract

GTP binding proteins of the Sec4/Ypt/rab family regulate distinct vesicular traffic events in eukaryotic cells. We have cloned GDI1, an essential homolog of bovine rab GDI (GDP dissociation inhibitor) from the yeast Saccharomyces cerevisiae. Analogous to the bovine protein, purified Gdi1p slows the dissociation of GDP from Sec4p and releases the GDP-bound form from yeast membranes. Depletion of Gdi1p in vivo leads to loss of the soluble pool of Sec4p and inhibition of protein transport at multiple stages of the secretory pathway. Complementation analysis indicates that GDI1 is allelic to sec19-1. These results establish that Gdi1p plays an essential function in membrane traffic and are consistent with a role for Gdi1p in the recycling of proteins of the Sec4/Ypt/rab family from their target membranes back to their vesicular pools.

Citing Articles

Mycoparasitism related targets of Tmk1 indicate stimulating regulatory functions of this MAP kinase in Trichoderma atroviride.

Atanasova L, Marchetti-Deschmann M, Nemes A, Bruckner B, Rehulka P, Stralis-Pavese N Sci Rep. 2023; 13(1):19976.

PMID: 37968441 PMC: 10651915. DOI: 10.1038/s41598-023-47027-6.

Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage.

Mashimo M, Morozumi A, Nobeyama A, Kanzaki M, Negi S, Kato J Int J Mol Sci. 2022; 23(14).

PMID: 35887176 PMC: 9319841. DOI: 10.3390/ijms23147827.

Mutant Presenilin 1 Dysregulates Exosomal Proteome Cargo Produced by Human-Induced Pluripotent Stem Cell Neurons.

Podvin S, Jones A, Liu Q, Aulston B, Mosier C, Ames J ACS Omega. 2021; 6(20):13033-13056.

PMID: 34056454 PMC: 8158845. DOI: 10.1021/acsomega.1c00660.

In-depth and 3-dimensional exploration of the budding yeast phosphoproteome.

Lanz M, Yugandhar K, Gupta S, Sanford E, Faca V, Vega S EMBO Rep. 2021; 22(2):e51121.

PMID: 33491328 PMC: 7857435. DOI: 10.15252/embr.202051121.

Conserved juxtamembrane domains in the yeast golgin Coy1 drive assembly of a megadalton-sized complex and mediate binding to tethering and SNARE proteins.

Anderson N, Barlowe C J Biol Chem. 2019; 294(25):9690-9705.

PMID: 31073031 PMC: 6597839. DOI: 10.1074/jbc.RA119.008107.

References

Smith D, Johnson K . Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988; 67(1):31-40. DOI: 10.1016/0378-1119(88)90005-4. View

Novick P, Garrett M, Brennwald P, Kabcenell A . Purification of Sec4 protein from Saccharomyces cerevisiae and Escherichia coli. Methods Enzymol. 1992; 219:352-62. DOI: 10.1016/0076-6879(92)19035-5. View

Hunkapiller M, Lujan E, Ostrander F, HOOD L . Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 1983; 91:227-36. DOI: 10.1016/s0076-6879(83)91019-4. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Dulic V, Egerton M, Elguindi I, Raths S, Singer B, Riezman H . Yeast endocytosis assays. Methods Enzymol. 1991; 194:697-710. DOI: 10.1016/0076-6879(91)94051-d. View

Goud B, Salminen A, Walworth N, Novick P . A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell. 1988; 53(5):753-68. DOI: 10.1016/0092-8674(88)90093-1. View

Ueda T, Kikuchi A, Ohga N, Yamamoto J, Takai Y . Purification and characterization from bovine brain cytosol of a novel regulatory protein inhibiting the dissociation of GDP from and the subsequent binding of GTP to rhoB p20, a ras p21-like GTP-binding protein. J Biol Chem. 1990; 265(16):9373-80. View

Gorvel J, Chavrier P, Zerial M, Gruenberg J . rab5 controls early endosome fusion in vitro. Cell. 1991; 64(5):915-25. DOI: 10.1016/0092-8674(91)90316-q. View

Chavrier P, Parton R, Hauri H, Simons K, Zerial M . Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell. 1990; 62(2):317-29. DOI: 10.1016/0092-8674(90)90369-p. View

10.

Rossi G, Yu J, Newman A . Dependence of Ypt1 and Sec4 membrane attachment on Bet2. Nature. 1991; 351(6322):158-61. DOI: 10.1038/351158a0. View

11.

Seabra M, Brown M, Slaughter C, Sudhof T, Goldstein J . Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell. 1992; 70(6):1049-57. DOI: 10.1016/0092-8674(92)90253-9. View

12.

Kaiser C, Schekman R . Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 1990; 61(4):723-33. DOI: 10.1016/0092-8674(90)90483-u. View

13.

Matsui Y, Kikuchi A, Araki S, Hata Y, Kondo J, Teranishi Y . Molecular cloning and characterization of a novel type of regulatory protein (GDI) for smg p25A, a ras p21-like GTP-binding protein. Mol Cell Biol. 1990; 10(8):4116-22. PMC: 360933. DOI: 10.1128/mcb.10.8.4116-4122.1990. View

14.

Jiang Y, Rossi G . Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature. 1993; 366(6450):84-6. DOI: 10.1038/366084a0. View

15.

Zahner J, Cheney C . A Drosophila homolog of bovine smg p25a GDP dissociation inhibitor undergoes a shift in isoelectric point in the developmental mutant quartet. Mol Cell Biol. 1993; 13(1):217-27. PMC: 358901. DOI: 10.1128/mcb.13.1.217-227.1993. View

16.

Bucci C, Parton R, Mather I, Stunnenberg H, Simons K, Hoflack B . The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell. 1992; 70(5):715-28. DOI: 10.1016/0092-8674(92)90306-w. View

17.

Garrett M, Kabcenell A, Zahner J, Kaibuchi K, Sasaki T, Takai Y . Interaction of Sec4 with GDI proteins from bovine brain, Drosophila melanogaster and Saccharomyces cerevisiae. Conservation of GDI membrane dissociation activity. FEBS Lett. 1993; 331(3):233-8. DOI: 10.1016/0014-5793(93)80343-s. View

18.

Potenza M, Bowser R, Muller H, Novick P . SEC6 encodes an 85 kDa soluble protein required for exocytosis in yeast. Yeast. 1992; 8(7):549-58. DOI: 10.1002/yea.320080706. View

19.

Rothman J, Orci L . Molecular dissection of the secretory pathway. Nature. 1992; 355(6359):409-15. DOI: 10.1038/355409a0. View

20.

Li R, Murray A . Feedback control of mitosis in budding yeast. Cell. 1991; 66(3):519-31. DOI: 10.1016/0092-8674(81)90015-5. View