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Recognition of Chimeric Small-subunit Ribosomal DNAs Composed of Genes from Uncultivated Microorganisms

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Date 1994 Feb 1
PMID 8135524
Citations 66
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Abstract

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

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