Developmentally Excised Sequences in Micronuclear DNA of Paramecium

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1994 Mar 15

PMID 8134383

Citations 25

Authors

C J Steele

G A Barkocy-Gallagher

L B Preer

J R PREER Jr

Affiliations

Soon will be listed here.

Abstract

DNA processing occurs in ciliates at autogamy and conjugation when new macronuclei are formed from micronuclei and old macronuclei degrade. Processing of micronuclear DNA consists of removal of certain internal sequences, chromosomal fragmentation, addition of new telomeres, and amplification. Aside from a recent brief report, internal eliminated sequences have not been described in Paramecium. In this paper we characterize nine internal eliminated sequences found within and near the gene that codes for surface protein A in Paramecium tetraurelia. Of these nine, seven are located within the translated portion of the gene, and all include short, inverted terminal repeats. The characteristic sequence, TA, appears at the boundaries of all of the internal eliminated sequences.

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