Localization of the 92 Kd Gelatinase MRNA in Squamous Cell and Adenocarcinomas of the Lung Using in Situ Hybridization

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1994 Mar 1

PMID 8129037

Citations 18

Authors

L Litzky

I Lubensky

R J Muschel

Affiliations

Soon will be listed here.

Abstract

We have used in situ hybridization for RNA to localize cells containing mRNA for the 92 kd gelatinase in carcinomas of the lung. We used archival material to analyze sections from 12 cases of squamous cell carcinomas of the lung including six stage I and three stage II and from three cases of adenocarcinoma of the lung. Presence of mRNA in the tissue was verified by in situ hybridization for gamma actin. The 92 kd gelatinase mRNA was found in all 12 squamous cell carcinomas tumors and was highly expressed in the tumor cells themselves. In addition, it was found in host stromal cells surrounding the tumor, but not in normal lung fibroblasts. In contrast it was not found in the adenocarcinomas of the lung or in the stroma surrounding these tumors. The mRNA for the 92 kd gelatinase was present in normal pulmonary tissue, bronchial epithelium, basal cell hyperplasia of bronchial epithelium, alveolar macrophages, and focally in bronchial mucous glands. It was not present in normal alveoli, vascular cells, cartilage, or most lymphocytes. We corroborated the presence of the mRNA for the 92 kd gelatinase by ribonuclease protection assay. The levels of mRNA for the 92 kd gelatinase in two specimens of squamous cell carcinoma were 6- to 10-fold greater than in the nonneoplastic tissue and two adenocarcinoma specimens.

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References

Sellers A, Murphy G, Meikle M, Reynolds J . Rabbit bone collagenase inhibitor blocks the activity of other neutral metalloproteinases. Biochem Biophys Res Commun. 1979; 87(2):581-7. DOI: 10.1016/0006-291x(79)91834-5. View

Wilhelm S, Collier I, Marmer B, Eisen A, Grant G, Goldberg G . SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages. J Biol Chem. 1989; 264(29):17213-21. View

Matrisian L, Bowden G, Krieg P, Furstenberger G, Briand J, Leroy P . The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than in benign tumors. Proc Natl Acad Sci U S A. 1986; 83(24):9413-7. PMC: 387148. DOI: 10.1073/pnas.83.24.9413. View

Murphy G, Koklitis P, Carne A . Dissociation of tissue inhibitor of metalloproteinases (TIMP) from enzyme complexes yields fully active inhibitor. Biochem J. 1989; 261(3):1031-4. PMC: 1138932. DOI: 10.1042/bj2611031. View

Goldberg G, Marmer B, Grant G, Eisen A, Wilhelm S, He C . Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2. Proc Natl Acad Sci U S A. 1989; 86(21):8207-11. PMC: 298249. DOI: 10.1073/pnas.86.21.8207. View

Monteagudo C, Merino M, Liotta L, Stetler-Stevenson W . Immunohistochemical distribution of type IV collagenase in normal, benign, and malignant breast tissue. Am J Pathol. 1990; 136(3):585-92. PMC: 1877499. View

Bernhard E, Muschel R, Hughes E . Mr 92,000 gelatinase release correlates with the metastatic phenotype in transformed rat embryo cells. Cancer Res. 1990; 50(13):3872-7. View

Okada Y, Tsuchiya H, Shimizu H, Tomita K, Nakanishi I, Sato H . Induction and stimulation of 92-kDa gelatinase/type IV collagenase production in osteosarcoma and fibrosarcoma cell lines by tumor necrosis factor alpha. Biochem Biophys Res Commun. 1990; 171(2):610-7. DOI: 10.1016/0006-291x(90)91190-4. View

Welgus H, Campbell E, Cury J, Eisen A, Senior R, Wilhelm S . Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development. J Clin Invest. 1990; 86(5):1496-502. PMC: 296895. DOI: 10.1172/JCI114867. View

10.

Basset P, Bellocq J, Wolf C, Stoll I, Hutin P, Limacher J . A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature. 1990; 348(6303):699-704. DOI: 10.1038/348699a0. View

11.

Levy A, Cioce V, Sobel M, Garbisa S, Grigioni W, Liotta L . Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 1991; 51(1):439-44. View

12.

Liotta L, Steeg P, Stetler-Stevenson W . Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell. 1991; 64(2):327-36. DOI: 10.1016/0092-8674(91)90642-c. View

13.

Librach C, Werb Z, Fitzgerald M, Chiu K, Corwin N, Esteves R . 92-kD type IV collagenase mediates invasion of human cytotrophoblasts. J Cell Biol. 1991; 113(2):437-49. PMC: 2288933. DOI: 10.1083/jcb.113.2.437. View

14.

Senior R, Griffin G, Fliszar C, Shapiro S, Goldberg G, Welgus H . Human 92- and 72-kilodalton type IV collagenases are elastases. J Biol Chem. 1991; 266(12):7870-5. View

15.

Muller D, Breathnach R, Engelmann A, Millon R, Bronner G, Flesch H . Expression of collagenase-related metalloproteinase genes in human lung or head and neck tumours. Int J Cancer. 1991; 48(4):550-6. DOI: 10.1002/ijc.2910480412. View

16.

Murphy G, Cockett M, WARD R, Docherty A . Matrix metalloproteinase degradation of elastin, type IV collagen and proteoglycan. A quantitative comparison of the activities of 95 kDa and 72 kDa gelatinases, stromelysins-1 and -2 and punctuated metalloproteinase (PUMP). Biochem J. 1991; 277 ( Pt 1):277-9. PMC: 1151220. DOI: 10.1042/bj2770277. View

17.

Matrisian L, Bowden G . Stromelysin/transin and tumor progression. Semin Cancer Biol. 1990; 1(2):107-15. View

18.

Liotta L, Stetler-Stevenson W . Metalloproteinases and cancer invasion. Semin Cancer Biol. 1990; 1(2):99-106. View

19.

Huhtala P, Tuuttila A, Chow L, Lohi J, Keski-Oja J, Tryggvason K . Complete structure of the human gene for 92-kDa type IV collagenase. Divergent regulation of expression for the 92- and 72-kilodalton enzyme genes in HT-1080 cells. J Biol Chem. 1991; 266(25):16485-90. View

20.

Unemori E, Hibbs M, Amento E . Constitutive expression of a 92-kD gelatinase (type V collagenase) by rheumatoid synovial fibroblasts and its induction in normal human fibroblasts by inflammatory cytokines. J Clin Invest. 1991; 88(5):1656-62. PMC: 295696. DOI: 10.1172/JCI115480. View