Time-dependent Diffusion of Water in a Biological Model System

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1994 Feb 15

PMID 8108392

Citations 141

Authors

L L Latour

K Svoboda

P P Mitra

C H Sotak

Affiliations

Soon will be listed here.

Abstract

Packed erythrocytes are ideally suited as a model system for the study of water diffusion in biological tissue, because cell size, membrane permeability, and extracellular volume fraction can be varied independently. We used a pulsed-field-gradient spin echo NMR technique to measure the time-dependent diffusion coefficient D(t) in packed erythrocytes. The long-time diffusion constant, D(eff), depends sensitively on the extracellular volume fraction. This may explain the drop in D(eff) during the early stages of brain ischemia, where just minutes after an ischemic insult the extra-cellular volume in the affected region of the brain is significantly reduced. Using an effective medium formula, we estimate the erythrocyte membrane permeability, in good agreement with measurements on isolated cells. From the short-time behavior of D(t), we determine the surface-to-volume ratio of the cells, approximately (0.72 micron)-1.

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References

Moseley M, Cohen Y, Mintorovitch J, Chileuitt L, Shimizu H, Kucharczyk J . Early detection of regional cerebral ischemia in cats: comparison of diffusion- and T2-weighted MRI and spectroscopy. Magn Reson Med. 1990; 14(2):330-46. DOI: 10.1002/mrm.1910140218. View

Solomon A . Water channels across the red blood cell and other biological membranes. Methods Enzymol. 1989; 173:192-222. DOI: 10.1016/s0076-6879(89)73013-5. View

Farmer R, MACEY R . Perturbation of red cell volume: rectification of osmotic flow. Biochim Biophys Acta. 1970; 196(1):53-65. DOI: 10.1016/0005-2736(70)90165-3. View

Redwood W, Rall E, PERL W . Red cell membrane permeability deduced from bulk diffusion coefficients. J Gen Physiol. 1974; 64(6):706-29. PMC: 2226182. DOI: 10.1085/jgp.64.6.706. View

MACEY R, Farmer R . Inhibition of water and solute permeability in human red cells. Biochim Biophys Acta. 1970; 211(1):104-6. DOI: 10.1016/0005-2736(70)90130-6. View

Schuier F, Hossmann K . Experimental brain infarcts in cats. II. Ischemic brain edema. Stroke. 1980; 11(6):593-601. DOI: 10.1161/01.str.11.6.593. View

Cooper R, Chang D, Young A, Martin C . Restricted diffusion in biophysical systems. Experiment. Biophys J. 1974; 14(3):161-77. PMC: 1334492. DOI: 10.1016/s0006-3495(74)85904-7. View

Svoboda K, Schmidt C, Branton D, Block S . Conformation and elasticity of the isolated red blood cell membrane skeleton. Biophys J. 1992; 63(3):784-93. PMC: 1262211. DOI: 10.1016/S0006-3495(92)81644-2. View

Le Bihan D, Turner R, Douek P, Patronas N . Diffusion MR imaging: clinical applications. AJR Am J Roentgenol. 1992; 159(3):591-9. DOI: 10.2214/ajr.159.3.1503032. View

10.

Mitra , Sen , Schwartz , Le Doussal P . Diffusion propagator as a probe of the structure of porous media. Phys Rev Lett. 1992; 68(24):3555-3558. DOI: 10.1103/PhysRevLett.68.3555. View

11.

Crick F . Diffusion in embryogenesis. Nature. 1970; 225(5231):420-2. DOI: 10.1038/225420a0. View

12.

Hajnal J, Doran M, Hall A, Collins A, Oatridge A, Pennock J . MR imaging of anisotropically restricted diffusion of water in the nervous system: technical, anatomic, and pathologic considerations. J Comput Assist Tomogr. 1991; 15(1):1-18. DOI: 10.1097/00004728-199101000-00001. View

13.

Tanner J . Intracellular diffusion of water. Arch Biochem Biophys. 1983; 224(2):416-28. DOI: 10.1016/0003-9861(83)90228-x. View

14.

Klosgen B, Schonert H, DEUTICKE B . Measurement of rapid membrane permeation in cell suspensions by application of a generalized capillary method. Biochim Biophys Acta. 1988; 939(1):29-39. DOI: 10.1016/0005-2736(88)90043-0. View

15.

Garcia J, Anderson M . Physiopathology of cerebral ischemia. Crit Rev Neurobiol. 1989; 4(4):303-24. View

16.

Knight R, Ordidge R, Helpern J, Chopp M, Rodolosi L, Peck D . Temporal evolution of ischemic damage in rat brain measured by proton nuclear magnetic resonance imaging. Stroke. 1991; 22(6):802-8. DOI: 10.1161/01.str.22.6.802. View

17.

Endre Z, Kuchel P, Chapman B . Cell volume dependence of 1H spin-echo NMR signals in human erythrocyte suspensions. The influence of in situ field gradients. Biochim Biophys Acta. 1984; 803(3):137-44. DOI: 10.1016/0167-4889(84)90003-x. View