Tandemly Repeated Transgenes of the Human Minisatellite MS32 (D1S8), with Novel Mouse Gamma Satellite Integration

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1994 Aug 11

PMID 8065909

Citations 10

Authors

M J Allen

A J Jeffreys

M A Surani

S Barton

M L Norris

A Collick

Affiliations

Soon will be listed here.

Abstract

The human hypervariable minisatellite MS32 has a well characterised internal repeat unit array and high mutation rates have been observed at this locus. Analysis of MS32 mutants has shown that male germline mutations are polarised to one end of the array and frequently involve complex gene conversion-like events, suggesting that tandem repeat instability may be modulated by cis-acting sequences flanking the locus. In order to investigate the processes affecting MS32 mutation rate and mechanism, we have created transgenic mice harbouring an MS32 allele. Here we describe the organisation of eight transgenic insertions. Analysis of these transgenic loci by MVR-PCR and structural analysis of the junctions between mouse flanking DNA and the transgenic loci has shed light on mechanisms of integration and rearrangement of the tandem repeated transgenes. Sequence analysis of the mouse DNA flanking these transgenes has shown that 5 of the 8 insertions have integrated into mouse gamma satellite repeated sequence. This suggests a non-random integration of the MS32 transgene construct into the mouse genome.

Citing Articles

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Mechanism of random integration of foreign DNA in transgenic mice.

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Lentiviral vector integration profiles differ in rodent postmitotic tissues.

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Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line.

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