Effect of Chromosomal Position on Amplification of Transfected Genes in Animal Cells

Overview

Journal Nature

Specialty Science

Date 1984 Feb 9

PMID 6694743

Citations 35

Authors

G M Wahl

B Robert de Saint Vincent

M L DeRose

Affiliations

Soon will be listed here.

Abstract

A single protein (CAD) contains the first three enzymatic activities of de novo uridine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.

Citing Articles

Advancements in Focal Amplification Detection in Tumor/Liquid Biopsies and Emerging Clinical Applications.

Arshadi A, Tolomeo D, Venuto S, Storlazzi C Genes (Basel). 2023; 14(6).

PMID: 37372484 PMC: 10298061. DOI: 10.3390/genes14061304.

Extrachromosomal Circular DNA from TCGA Tumors Is Generated from Common Genomic Loci, Is Characterized by Self-Homology and DNA Motifs near Circle Breakpoints.

Tatman P, Black J Cancers (Basel). 2022; 14(9).

PMID: 35565439 PMC: 9101409. DOI: 10.3390/cancers14092310.

Extrachromosomal Circular DNA (eccDNA): From Chaos to Function.

Zuo S, Yi Y, Wang C, Li X, Zhou M, Peng Q Front Cell Dev Biol. 2022; 9:792555.

PMID: 35083218 PMC: 8785647. DOI: 10.3389/fcell.2021.792555.

Novel insights into extrachromosomal DNA: redefining the onco-drivers of tumor progression.

Gu X, Yu J, Chai P, Ge S, Fan X J Exp Clin Cancer Res. 2020; 39(1):215.

PMID: 33046109 PMC: 7552444. DOI: 10.1186/s13046-020-01726-4.

Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection.

Steinrueck M, Guet C Elife. 2017; 6.

PMID: 28738969 PMC: 5526668. DOI: 10.7554/eLife.25100.