Lysophosphoinositide-specific Phospholipase C in Rat Brain Synaptic Plasma Membranes

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1994 Apr 1

PMID 8065496

Citations 8

Authors

T Tsutsumi

T Kobayashi

H Ueda

E Yamauchi

S Watanabe

H Okuyama

Affiliations

Soon will be listed here.

Abstract

A membrane preparation from rat brain catalyzed the hydrolysis of [2-3H]glycerol-labeled lysophosphatidylinositol (lysoPI) to yield monoacylglycerol (MG) and inositolphosphates. This phospholipase C activity had an optimal pH of 8.2. The membrane preparation did not require the addition of Ca2+ for its maximum activity, but the activity was inhibited by addition of 0.1 mM EDTA to the assay mixture and was restored by simultaneous addition of 0.2 mM Ca2+. The activity was found to be localized in synaptic plasma membranes prepared by Ficoll and Percoll density gradients. The phospholipase C was highly specific for lysoPI; diacylglycerol formation from phosphatidylinositol, and MG formation from lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine were below 5% of that observed with lysoPI under the conditions used. We concluded that there is a pathway for phosphatidylinositol metabolism in brain synaptic membranes which is different from the well-characterized phosphoinositide-specific phospholipase C pathway.

Citing Articles

From Classical to Alternative Pathways of 2-Arachidonoylglycerol Synthesis: AlterAGs at the Crossroad of Endocannabinoid and Lysophospholipid Signaling.

Briand-Mesange F, Gennero I, Salles J, Trudel S, Dahan L, Ausseil J Molecules. 2024; 29(15).

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Glycerophosphodiesterase 3 (GDE3) is a lysophosphatidylinositol-specific ectophospholipase C acting as an endocannabinoid signaling switch.

Briand-Mesange F, Pons V, Allart S, Masquelier J, Chicanne G, Beton N J Biol Chem. 2020; 295(46):15767-15781.

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Fatty Acid modulation of the endocannabinoid system and the effect on food intake and metabolism.

Naughton S, Mathai M, Hryciw D, McAinch A Int J Endocrinol. 2013; 2013:361895.

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Multiple functions of endocannabinoid signaling in the brain.

Katona I, Freund T Annu Rev Neurosci. 2012; 35:529-58.

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Purification and characterization of lysophospholipase C from pig brain.

Hung N, Kim M, Sok D Neurochem Res. 2009; 35(1):50-9.

PMID: 19588246 DOI: 10.1007/s11064-009-0029-2.

References

Homma Y, Imaki J, Nakanishi O, Takenawa T . Isolation and characterization of two different forms of inositol phospholipid-specific phospholipase C from rat brain. J Biol Chem. 1988; 263(14):6592-8. View

Nakao T, Nakao M, Mizuno N, Komatsu Y, Fujita M . Purification and some properties of Na,K-transport ATPase. I. Solubilization and stability of Lubrol extracts. J Biochem. 1973; 73(3):609-19. DOI: 10.1093/oxfordjournals.jbchem.a130119. View

Ueda H, Kobayashi T, Kishimoto M, Tsutsumi T, Okuyama H . A possible pathway of phosphoinositide metabolism through EDTA-insensitive phospholipase A1 followed by lysophosphoinositide-specific phospholipase C in rat brain. J Neurochem. 1993; 61(5):1874-81. DOI: 10.1111/j.1471-4159.1993.tb09829.x. View

Hirasawa K, Irvine R, Dawson R . The catabolism of phosphatidylinisitol by an EDTA-insensitive phospholipase A1 and calcium-dependent phosphatidylinositol phosphodiesterase in rat brain. Eur J Biochem. 1981; 120(1):53-8. DOI: 10.1111/j.1432-1033.1981.tb05669.x. View

Murase S, Yamada K, Naito Y, Hirose J, Okuyama H . Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. J Biochem. 1988; 104(2):270-5. DOI: 10.1093/oxfordjournals.jbchem.a122456. View

Rhee S, Choi K . Regulation of inositol phospholipid-specific phospholipase C isozymes. J Biol Chem. 1992; 267(18):12393-6. View

Fox J, Soliz N, Saltiel A . Purification of a phosphatidylinositol-glycan-specific phospholipase C from liver plasma membranes: a possible target of insulin action. Proc Natl Acad Sci U S A. 1987; 84(9):2663-7. PMC: 304718. DOI: 10.1073/pnas.84.9.2663. View

Ryu S, Cho K, Lee K, Suh P, Rhee S . Purification and characterization of two immunologically distinct phosphoinositide-specific phospholipases C from bovine brain. J Biol Chem. 1987; 262(26):12511-8. View

NISHIZUKA Y . The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988; 334(6184):661-5. DOI: 10.1038/334661a0. View

10.

Okuyama H, WAKIL S . Positional specificities of acyl coenzyme A: glycerophosphate and acyl coenzyme A: monoacylglycerophosphate acyltransferases in Escherichia coli. J Biol Chem. 1973; 248(14):5197-205. View

11.

Rhee S, Suh P, Ryu S, Lee S . Studies of inositol phospholipid-specific phospholipase C. Science. 1989; 244(4904):546-50. DOI: 10.1126/science.2541501. View

12.

Doi O, Nojima S . Lysophospholipase of Escherichia coli. J Biol Chem. 1975; 250(13):5208-14. View

13.

Heymann D, Reddington M, Kreutzberg G . Subcellular localization of 5'-nucleotidase in rat brain. J Neurochem. 1984; 43(4):971-8. DOI: 10.1111/j.1471-4159.1984.tb12832.x. View

14.

Takenawa T, Homma Y, Emori Y . Properties of phospholipase C isozymes. Methods Enzymol. 1991; 197:511-8. DOI: 10.1016/0076-6879(91)97177-z. View

15.

Hiles I, Otsu M, Volinia S, Fry M, Gout I, Dhand R . Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit. Cell. 1992; 70(3):419-29. DOI: 10.1016/0092-8674(92)90166-a. View

16.

Comfurius P, ZWAAL R . The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography. Biochim Biophys Acta. 1977; 488(1):36-42. DOI: 10.1016/0005-2760(77)90120-5. View

17.

Cockcroft S, Thomas G . Inositol-lipid-specific phospholipase C isoenzymes and their differential regulation by receptors. Biochem J. 1992; 288 ( Pt 1):1-14. PMC: 1132071. DOI: 10.1042/bj2880001. View

18.

Bjerve K, Daae L, Bremer J . The selective loss of lysophospholipids in some commonly used lipid-extraction procedures. Anal Biochem. 1974; 58(1):238-45. DOI: 10.1016/0003-2697(74)90463-1. View

19.

Murase S, Okuyama H . A membrane-bound phospholipase C with an apparent specificity for lysophosphatidylinositol in porcine platelets. J Biol Chem. 1985; 260(1):262-5. View

20.

Rendon A, Masmoudi A . Purification of non-synaptic and synaptic mitochondria and plasma membranes from rat brain by a rapid Percoll gradient procedure. J Neurosci Methods. 1985; 14(1):41-51. DOI: 10.1016/0165-0270(85)90113-x. View