Energy Metabolism in Leishmania

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1994 Apr 1

PMID 8056781

Citations 7

Authors

J J Blum

Affiliations

Soon will be listed here.

Abstract

Alanine plays a key role in the response of promastigotes to osmotic stress and to hypoxia. It is rapidly released in response to hypo-osmolality, is consumed from its large intracellular pool under iso-osmotic conditions even in the presence of glucose, and is synthesized under hyperosmotic conditions even in the absence of glucose. Its rate of oxidation, in the presence or absence of any of ten other amino acids tested, is strongly inhibited by hyperosmolality. Glucose oxidation is also inhibited by hyperosmolality, but to a lesser extent than that of alanine, and is inhibited by alanine, glutamate, and aspartate. Hyperosmolality also inhibits the incorporation of label from [2-14C]acetate into the putative storage carbohydrate, mannan, which occurs via the glyoxylate bypass and the as yet unexplored "mannoneogenic" pathway. The rates of glycolysis and of oxidation of several amino acids decrease with increasing culture age, but the capacity to oxidize fatty acids increases, and in cells from 3-day stationary phase cultures hyperosmolality enhances rather than inhibits alanine oxidation.

Citing Articles

Uptake of l-Alanine and Its Distinct Roles in the Bioenergetics of Trypanosoma cruzi.

Girard R, Crispim M, Alencar M, Silber A mSphere. 2018; 3(4).

PMID: 30021880 PMC: 6052336. DOI: 10.1128/mSphereDirect.00338-18.

Hypoxia, Hypoxia-Inducible Factor-1α, and Innate Antileishmanial Immune Responses.

Schatz V, Neubert P, Rieger F, Jantsch J Front Immunol. 2018; 9:216.

PMID: 29520262 PMC: 5827161. DOI: 10.3389/fimmu.2018.00216.

Inhibition of fumarate reductase in Leishmania major and L. donovani by chalcones.

Chen M, Zhai L, Christensen S, Theander T, Kharazmi A Antimicrob Agents Chemother. 2001; 45(7):2023-9.

PMID: 11408218 PMC: 90595. DOI: 10.1128/AAC.45.7.2023-2029.2001.

Compartmentation protects trypanosomes from the dangerous design of glycolysis.

Bakker B, Mensonides F, Teusink B, van Hoek P, Michels P, Westerhoff H Proc Natl Acad Sci U S A. 2000; 97(5):2087-92.

PMID: 10681445 PMC: 15758. DOI: 10.1073/pnas.030539197.

Elucidation of carbon sources used for the biosynthesis of fatty acids and sterols in the trypanosomatid Leishmania mexicana.

Ginger M, Chance M, Goad L Biochem J. 1999; 342 ( Pt 2):397-405.

PMID: 10455027 PMC: 1220477.

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