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Isolation of Human Eosinophils and Their Lymphocyte-like Rosetting Properties

Overview
Journal Immunology
Specialty Allergy & Immunology
Date 1976 Sep 1
PMID 800403
Citations 10
Authors
R SHER
A Glover
Affiliations
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Abstract

A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.

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An improved method for the isolation of eosinophilic leukocytes from normal human blood.

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