A Luciferase Expression System for Physarum That Facilitates Analysis of Regulatory Elements

Overview

Journal Curr Genet

Specialty Genetics

Date 1994 Aug 1

PMID 8001165

Citations 2

Authors

J Bailey

M Benard

T G Burland

Affiliations

Soon will be listed here.

Abstract

We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.

Citing Articles

The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter.

Pierron G, Pallotta D, Benard M Mol Cell Biol. 1999; 19(5):3506-14.

PMID: 10207074 PMC: 84143. DOI: 10.1128/MCB.19.5.3506.

Homologous gene replacement in Physarum.

Burland T, Pallotta D Genetics. 1995; 139(1):147-58.

PMID: 7705620 PMC: 1206314. DOI: 10.1093/genetics/139.1.147.

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