Transient Expression in Physarum of a Chloramphenicol Acetyltransferase Gene Under the Control of Actin Gene Promoters

Overview

Journal Curr Genet

Specialty Genetics

Date 1992 Apr 1

PMID 1525865

Citations 7

Authors

T G Burland

J Bailey

L Adam

M J Mukhopadhyay

W F Dove

D Pallotta

Affiliations

Soon will be listed here.

Abstract

We cloned and sequenced two actin promoters from Physarum, and constructed plasmids carrying these promoters upstream of a bacterial chloramphenicol acetyltransferase (cat) gene. We then tested the plasmids for their ability to express cat in Physarum amoebae. We present reliable methods for introducing plasmid DNA into Physarum amoebae by electroporation, and show that expression of the cat gene in amoebae occurs in the presence, but not the absence, of one or the other Physarum actin promoter.

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