The Origin of Human Chromosome 2 Analyzed by Comparative Chromosome Mapping with a DNA Microlibrary

Overview

Journal Chromosome Res

Date 1994 Sep 1

PMID 7981945

Citations 19

Authors

J Wienberg

A Jauch

H J Ludecke

G Senger

B Horsthemke

U Claussen

T Cremer

N Arnold

C Lengauer

Affiliations

Soon will be listed here.

Abstract

Fluorescence in situ hybridization (FISH) of microlibraries established from distinct chromosome subregions can test the evolutionary conservation of chromosome bands as well as chromosomal rearrangements that occurred during primate evolution and will help to clarify phylogenetic relationships. We used a DNA library established by microdissection and microcloning from the entire long arm of human chromosome 2 for fluorescence in situ hybridization and comparative mapping of the chromosomes of human, great apes (Pan troglodytes, Pan paniscus, Gorilla gorilla, Pongo pygmaeus) and Old World monkeys (Macaca fuscata and Cercopithecus aethiops). Inversions were found in the pericentric region of the primate chromosome 2p homologs in great apes, and the hybridization pattern demonstrates the known phylogenetically derived telomere fusion in the line that leads to human chromosome 2. The hybridization of the 2q microlibrary to chromosomes of Old World monkeys gave a different pattern from that in the gorilla and the orang-utan, but a pattern similar to that of chimpanzees. This suggests convergence of chromosomal rearrangements in different phylogenetic lines.

Citing Articles

Dark Matter of Primate Genomes: Satellite DNA Repeats and Their Evolutionary Dynamics.

Ahmad S, Singchat W, Jehangir M, Suntronpong A, Panthum T, Malaivijitnond S Cells. 2020; 9(12).

PMID: 33352976 PMC: 7767330. DOI: 10.3390/cells9122714.

The spindle assembly checkpoint and speciation.

Jackson R, Mistry H PeerJ. 2020; 8:e9073.

PMID: 32435535 PMC: 7224227. DOI: 10.7717/peerj.9073.

Evolutionary Dynamics of the POTE Gene Family in Human and Nonhuman Primates.

Maggiolini F, Mercuri L, Antonacci F, Anaclerio F, Calabrese F, Lorusso N Genes (Basel). 2020; 11(2).

PMID: 32085667 PMC: 7073761. DOI: 10.3390/genes11020213.

One pedigree we all may have come from - did Adam and Eve have the chromosome 2 fusion?.

Stankiewicz P Mol Cytogenet. 2016; 9:72.

PMID: 27708712 PMC: 5037601. DOI: 10.1186/s13039-016-0283-3.

Hominoid fission of chromosome 14/15 and the role of segmental duplications.

Giannuzzi G, Pazienza M, Huddleston J, Antonacci F, Malig M, Vives L Genome Res. 2013; 23(11):1763-73.

PMID: 24077392 PMC: 3814877. DOI: 10.1101/gr.156240.113.

References

Stanyon R, Arnold N, Koehler U, Bigoni F, Wienberg J . Chromosomal painting shows that "marked chromosomes" in lesser apes and Old World monkeys are not homologous and evolved by convergence. Cytogenet Cell Genet. 1995; 68(1-2):74-8. DOI: 10.1159/000133894. View

Avarello R, Pedicini A, Caiulo A, Zuffardi O, Fraccaro M . Evidence for an ancestral alphoid domain on the long arm of human chromosome 2. Hum Genet. 1992; 89(2):247-9. DOI: 10.1007/BF00217134. View

Baldini A, Ried T, Shridhar V, Ogura K, DAiuto L, Rocchi M . An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13. Hum Genet. 1993; 90(6):577-83. DOI: 10.1007/BF00202474. View

Lichter P, Cremer T, Borden J, Manuelidis L, Ward D . Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet. 1988; 80(3):224-34. DOI: 10.1007/BF01790090. View

Ried T, Lengauer C, Cremer T, Wiegant J, Raap A, VAN DER PLOEG M . Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization. Genes Chromosomes Cancer. 1992; 4(1):69-74. DOI: 10.1002/gcc.2870040109. View

Trautmann U, Leuteritz G, Senger G, Claussen U, Ballhausen W . Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe. Hum Genet. 1991; 87(4):495-7. DOI: 10.1007/BF00197175. View

Wells R, Germino G, Krishna S, Buckle V, Reeders S . Telomere-related sequences at interstitial sites in the human genome. Genomics. 1990; 8(4):699-704. DOI: 10.1016/0888-7543(90)90257-u. View

Wienberg J, Stanyon R, Jauch A, Cremer T . Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries. Chromosoma. 1992; 101(5-6):265-70. DOI: 10.1007/BF00346004. View

Klever M, Grond-Ginsbach C, Scherthan H . Chromosomal in situ suppression hybridization after Giemsa banding. Hum Genet. 1991; 86(5):484-6. DOI: 10.1007/BF00194638. View

10.

Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J . Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci U S A. 1988; 85(23):9138-42. PMC: 282679. DOI: 10.1073/pnas.85.23.9138. View

11.

Jauch A, Wienberg J, Stanyon R, Arnold N, Tofanelli S, Ishida T . Reconstruction of genomic rearrangements in great apes and gibbons by chromosome painting. Proc Natl Acad Sci U S A. 1992; 89(18):8611-5. PMC: 49970. DOI: 10.1073/pnas.89.18.8611. View

12.

Arnold N, Wienberg J, Ermert K, Zachau H . Comparative mapping of DNA probes derived from the V kappa immunoglobulin gene regions on human and great ape chromosomes by fluorescence in situ hybridization. Genomics. 1995; 26(1):147-50. DOI: 10.1016/0888-7543(95)80095-4. View

13.

Djalali M, Steinbach P, Bullerdiek J, HOLMES-SIEDLE M, Smith A . The significance of pericentric inversions of chromosome 2. Hum Genet. 1986; 72(1):32-6. DOI: 10.1007/BF00278814. View

14.

Lengauer C, Riethman H, Speicher M, Taniwaki M, Konecki D, Green E . Metaphase and interphase cytogenetics with Alu-PCR-amplified yeast artificial chromosome clones containing the BCR gene and the protooncogenes c-raf-1, c-fms, and c-erbB-2. Cancer Res. 1992; 52(9):2590-6. View

15.

Dutrillaux B . Chromosomal evolution in primates: tentative phylogeny from Microcebus murinus (Prosimian) to man. Hum Genet. 1979; 48(3):251-314. DOI: 10.1007/BF00272830. View

16.

Cremer T, Lichter P, Borden J, Ward D, Manuelidis L . Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes. Hum Genet. 1988; 80(3):235-46. DOI: 10.1007/BF01790091. View

17.

Wienberg J, Jauch A, Stanyon R, Cremer T . Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization. Genomics. 1990; 8(2):347-50. DOI: 10.1016/0888-7543(90)90292-3. View

18.

Ludecke H, Senger G, Claussen U, Horsthemke B . Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature. 1989; 338(6213):348-50. DOI: 10.1038/338348a0. View

19.

Gelman-Kohan Z, Rosensaft J, CHEMKE J . Homozygosity for inversion (2)(p12q14). Hum Genet. 1993; 92(4):427. DOI: 10.1007/BF01247351. View

20.

Yunis J, Prakash O . The origin of man: a chromosomal pictorial legacy. Science. 1982; 215(4539):1525-30. DOI: 10.1126/science.7063861. View