A System for Tissue-specific Gene Targeting: Transgenic Mice Susceptible to Subgroup A Avian Leukosis Virus-based Retroviral Vectors

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1994 Nov 8

PMID 7972042

Citations 66

Authors

M J Federspiel

P Bates

J A Young

H E Varmus

S H Hughes

Affiliations

Soon will be listed here.

Abstract

Avian leukosis viruses (ALVs) have been used extensively as genetic vectors in avian systems, but their utility in mammals or mammalian cell lines is compromised by inefficient viral entry. We have overcome this limitation by generating transgenic mice that express the receptor for the subgroup A ALV under the control of the chicken alpha sk-actin promoter. The skeletal muscles of these transgenic animals are susceptible to efficient infection by subgroup A ALV. Because infection is restricted to cell lineages that express the transgene, the method has utility for studies of development and oncogenesis and will provide models for tissue-specific gene therapy.

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