Lectin-induced Increase in Microvascular Permeability to Colloidal Carbon in Vitro May Involve Protein Kinase C Activation

Overview

Journal Agents Actions

Specialty Pharmacology

Date 1994 May 1

PMID 7942320

Citations 6

Authors

A M Northover

B J Northover

Affiliations

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Abstract

Two plant lectins, wheat germ agglutinin (WGA) and concanavalin A (Con A), which are known to bind to endothelial cells (ECs), were found to increase the leakage of colloidal carbon (CC) into the walls of microvessels in the villi of rat small intestine, when added to a gelatin-containing perfusate (GPSS) at a concentration of 10 micrograms/ml. Pretreatment of the microvessels with the protein kinase C (PKC) inhibitor Ro 31-8220 (1 x 10(-6) M) significantly reduced this effect. In contrast, the leakage of CC in response to A23187 (1 x 10(-4) M) was not affected by Ro 31-8220. Peanut agglutinin (PNA) and succinyl concanavalin A (SuccCon A), which do not bind to ECs, had no effect at a concentration of 10 micrograms/ml. A lower concentration of WGA (1 microgram/ml) had no significant effect of its own, but significantly reduced the leakage of CC in response to both platelet-activating factor (PAF, 5 x 10(-6) M) and 5-hydroxytryptamine (5-HT, 1 x 10(-4) M), but not to beta-phorbol 12,13-dibutyrate (PDB, 1 x 10(-6) M). These results suggest that all these effects of WGA and Con A involve cell surface receptors, albeit in a non-specific way. A possible mode of action is discussed.

Citing Articles

Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains.

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Crystallization and characterization of an inflammatory lectin purified from the seeds of Dioclea wilsonii.

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Vasodilator effects of Diocleinae lectins from the Canavalia genus.

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Rate of perfusion modulates colloidal carbon leakage from rat intestinal microvessels in vitro.

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Stimulation of protein kinase C activity may increase microvascular permeability to colloidal carbon via alpha-isoenzyme.

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PMID: 7843793 DOI: 10.1007/BF01560695.

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