Delayed-type Hypersensitivity Response in an Isogenic Murine Model of Paracoccidioidomycosis

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 1994 Jun 1

PMID 7935728

Citations 14

Authors

R dos A Fazioli

S S Kashino

E Burger

M F de Franco

V L Calich

Affiliations

Soon will be listed here.

Abstract

The specific delayed-type hypersensitivity (DTH) response was evaluated in resistant (A/SN) and susceptible (B10.A) mice intraperitoneally infected with yeasts from a virulent (Pb18) or from a non-virulent (Pb265) Paracoccidioides brasiliensis isolates. Both strains of mice were footpad challenged with homologous antigens. Pb18 infected A/SN mice developed an evident and persistent DTH response late in the course of the disease (90th day on) whereas B10.A animals mounted a discrete and ephemeral DTH response at the 14th day post-infection. A/SN mice infected with Pb265 developed cellular immune responses whereas B10.A mice were almost always anergic. Histological analysis of the footpads of infected mice at 48 hours after challenge showed a mixed infiltrate consisting of predominantly mononuclear cells. Previous infection of resistant and susceptible mice with Pb18 did not alter their DTH responses against heterologous unrelated antigens (sheep red blood cells and dinitrofluorobenzene) indicating that the observed cellular anergy was antigen-specific. When fungal related antigens (candidin and histoplasmin) were tested in resistant mice, absence of cross-reactivity was noted. Thus, specific DTH responses against P. brasiliensis depend on both the host's genetically determined resistance and the virulence of the fungal isolate.

Citing Articles

Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation.

Sperandio F, Fernandes G, Mendes A, Bani G, Calich V, Burger E Mediators Inflamm. 2016; 2015:430525.

PMID: 26819497 PMC: 4706933. DOI: 10.1155/2015/430525.

In pulmonary paracoccidioidomycosis IL-10 deficiency leads to increased immunity and regressive infection without enhancing tissue pathology.

Costa T, Bazan S, Feriotti C, Araujo E, Bassi E, Loures F PLoS Negl Trop Dis. 2013; 7(10):e2512.

PMID: 24205424 PMC: 3812093. DOI: 10.1371/journal.pntd.0002512.

B cell-deficient mice display enhanced susceptibility to Paracoccidioides brasiliensis Infection.

Tristao F, Panagio L, Rocha F, Cavassani K, Moreira A, Rossi M Mycopathologia. 2013; 176(1-2):1-10.

PMID: 23765323 DOI: 10.1007/s11046-013-9671-y.

Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.

Pina A, de Araujo E, Felonato M, Loures F, Feriotti C, Bernardino S Infect Immun. 2013; 81(4):1064-77.

PMID: 23340311 PMC: 3639589. DOI: 10.1128/IAI.00736-12.

CD28 exerts protective and detrimental effects in a pulmonary model of paracoccidioidomycosis.

Felonato M, Pina A, Bernardino S, Loures F, de Araujo E, Calich V Infect Immun. 2010; 78(11):4922-35.

PMID: 20713624 PMC: 2976317. DOI: 10.1128/IAI.00297-10.

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