Metallothionein MRNA in the Testis and Prostate of the Rat Detected by Digoxigenin-labeled Riboprobe

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1994 Jun 1

PMID 7928417

Citations 7

Authors

C Tohyama

N Nishimura

J S Suzuki

M Karasawa

H Nishimura

Affiliations

Soon will be listed here.

Abstract

Metallothionein (MT), a cysteine-rich heavy metal-binding protein, has been considered to play a role in the homeostatic control and detoxification of heavy metals, such as zinc, copper, and cadmium. In the present study, we have utilized a digoxigenin-labeled riboprobe to localize MT mRNA only by bright-field optics in the testis and prostate of the rat. In the rat testis, MT mRNA was found predominantly in primary spermatocytes and also in secondary spermatocytes and spermatids, but not in the spermatogonia, Sertoli cells, and Leydig cells. On the other hand, MT protein was present in these spermatogenic cells as well as in spermatozoa and Sertoli cells. In the prostate, MT mRNA was found predominantly in the epithelium of the dorsolateral lobes, but not in the ventral lobe, which is in agreement with the observed localization of MT protein. The utilization of both in situ hybridization and immunohistochemical staining on the same tissue specimens show MT gene expression in specific cell types in the male genital organs.

Citing Articles

Role of metallothionein-like cadmium-binding protein (MTLCdBP) in the protective mechanism against cadmium toxicity in the testis.

Ohta H, Qi Y, Ohba K, Toyooka T, Wang R Ind Health. 2019; 57(5):570-579.

PMID: 30606898 PMC: 6783288. DOI: 10.2486/indhealth.2018-0177.

A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells.

Berberian M, Pocognoni C, Mayorga L Int J Nanomedicine. 2018; 13:8075-8086.

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Unravelling the Role of Metallothionein on Development, Reproduction and Detoxification in the Wall Lizard Podarcis sicula.

Scudiero R, Verderame M, Motta C, Simoniello P Int J Mol Sci. 2017; 18(7).

PMID: 28753953 PMC: 5536057. DOI: 10.3390/ijms18071569.

Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure.

Verderame M, Limatola E, Scudiero R Eur J Histochem. 2017; 61(2):2777.

PMID: 28735517 PMC: 5641668. DOI: 10.4081/ejh.2017.2777.

Differential gene-expression of metallothionein 1M and 1G in response to zinc in sertoli TM4 cells.

Kheradmand F, Nourmohammadi I, Modarressi M, Firoozrai M, Ahmadi-Faghih M Iran Biomed J. 2010; 14(1-2):9-15.

PMID: 20683493 PMC: 3878141.

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