Immunohistochemical Localization of Metallothionein in the Eye of Rats

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1991 Jan 1

PMID 1856106

Citations 7

Authors

H Nishimura

N Nishimura

S Kobayashi

C Tohyama

Affiliations

Soon will be listed here.

Abstract

In order to elucidate possible physiological roles of metallothionein (MT), we have studied immunohistological localization of MT in the eye of the rat, using an avidin-biotin peroxidase complex method. As a result, strong MT immunostaining was observed in the epithelium of the lens and cornea. In the retina, considerably strong MT immunostaining was observed in the pigment cell layer while the nerve fiber layer and inner plexiform layer showed weak MT staining. Glial cells in the optic nerve were found to have marked MT staining. The present result is consistent with the hypothesis that MT may be involved not only in activation of zinc enzymes and cell proliferation through supply of zinc ions, but also in a protective mechanism in the blood-retina barrier.

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