Suppression of a Yeast Mitochondrial RNA Processing Defect by Nuclear Mutations

Overview

Journal Curr Genet

Specialty Genetics

Date 1994 Mar 1

PMID 7923410

Citations 1

Authors

P M Smooker

I J Macreadie

J L Wright

H B Lukins

Affiliations

Soon will be listed here.

Abstract

The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperature, 36 degrees C. This communication reports the isolation and characterization of several strains carrying nuclear mutations which suppress the temperature-sensitivity of h56. Both dominant and recessive suppressor mutations were isolated. One dominant suppressor strain (h56-S4) was characterized biochemically, and the mechanism of suppression shown to involve a restoration of precursor RNA processing at the restrictive temperature, with a concomitant increase in the synthesis of the var1 protein. It appears likely that the suppressing allele encodes a component of an RNA processing endoribonuclease active on var1 transcripts. A genomic library was constructed from the h56-S4 strain, and several plasmids showing suppressed activity were isolated. A preliminary analysis of these plasmids is presented.

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References

Murphy M, Choo K, Macreadie I, Marzuki S, Lukins H, Nagley P . Biogenesis of mitochondria: a temperature sensitivity mutation affecting the mitochondrially synthesized var1 protein of Saccharomyces cerevisiae. Arch Biochem Biophys. 1980; 203(1):260-70. DOI: 10.1016/0003-9861(80)90176-9. View

Smooker P, Wright J, Linnane A, Lukins H . A mitochondrial intergenic mutation affecting processing of specific yeast mitochondrial transcripts. Nucleic Acids Res. 1988; 16(19):9081-95. PMC: 338693. DOI: 10.1093/nar/16.19.9081. View

Schmelzer C, Schmidt C, May K, Schweyen R . Determination of functional domains in intron bI1 of yeast mitochondrial RNA by studies of mitochondrial mutations and a nuclear suppressor. EMBO J. 1983; 2(11):2047-52. PMC: 555407. DOI: 10.1002/j.1460-2075.1983.tb01698.x. View

Butow R, Perlman P, Grossman L . The unusual varl gene of yeast mitochondrial DNA. Science. 1985; 228(4707):1496-501. DOI: 10.1126/science.2990030. View

Nagley P, Linnane A . Expression of mitochondrial DNA in Saccharomyces cerevisiae: the construction of sets of isonuclear haploid strains containing different specified mitochondrial genomes. Biochem Biophys Res Commun. 1978; 85(2):585-92. DOI: 10.1016/0006-291x(78)91203-2. View

Dieckmann C, Homison G, Tzagoloff A . Assembly of the mitochondrial membrane system. Nucleotide sequence of a yeast nuclear gene (CBP1) involved in 5' end processing of cytochrome b pre-mRNA. J Biol Chem. 1984; 259(8):4732-8. View

Linder P, SLONIMSKI P . An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. Proc Natl Acad Sci U S A. 1989; 86(7):2286-90. PMC: 286897. DOI: 10.1073/pnas.86.7.2286. View

Lisowsky T, Michaelis G . Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae. Mol Gen Genet. 1989; 219(1-2):125-8. DOI: 10.1007/BF00261167. View

Klebe R, Harriss J, Sharp Z, DOUGLAS M . A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene. 1983; 25(2-3):333-41. DOI: 10.1016/0378-1119(83)90238-x. View

10.

Boy-Marcotte E, Jacquet M . A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant. Gene. 1982; 20(3):433-39. DOI: 10.1016/0378-1119(82)90212-8. View

11.

Costanzo M, Fox T . Control of mitochondrial gene expression in Saccharomyces cerevisiae. Annu Rev Genet. 1990; 24:91-113. DOI: 10.1146/annurev.ge.24.120190.000515. View

12.

Chang D, Clayton D . A mammalian mitochondrial RNA processing activity contains nucleus-encoded RNA. Science. 1987; 235(4793):1178-84. DOI: 10.1126/science.2434997. View

13.

Laughon A, GESTELAND R . Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast. Proc Natl Acad Sci U S A. 1982; 79(22):6827-31. PMC: 347226. DOI: 10.1073/pnas.79.22.6827. View

14.

Grivell L . Nucleo-mitochondrial interactions in yeast mitochondrial biogenesis. Eur J Biochem. 1989; 182(3):477-93. DOI: 10.1111/j.1432-1033.1989.tb14854.x. View

15.

Koll H, Schmidt C, Wiesenberger G, Schmelzer C . Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number. Curr Genet. 1987; 12(7):503-9. DOI: 10.1007/BF00419559. View

16.

JOHNSTON J, MORTIMER R . Use of snail digestive juice in isolation of yeast spore tetrads. J Bacteriol. 1959; 78:292. PMC: 290529. DOI: 10.1128/jb.78.2.292-292.1959. View

17.

Olson M, Loughney K, Hall B . Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci. J Mol Biol. 1979; 132(3):387-410. DOI: 10.1016/0022-2836(79)90267-5. View

18.

Zassenhaus H, Perlman P . Respiration deficient mutants in the A+T-rich region on yeast mitochondrial DNA containing the var1 gene. Curr Genet. 2013; 6(3):179-88. DOI: 10.1007/BF00390336. View

19.

Perlman P, Zhu H, Butow R . The nuclear SUV3-1 mutation affects a variety of post-transcriptional processes in yeast mitochondria. Nucleic Acids Res. 1990; 18(6):1369-76. PMC: 330499. DOI: 10.1093/nar/18.6.1369. View

20.

Bousquet I, Dujardin G, SLONIMSKI P . ABC1, a novel yeast nuclear gene has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex. EMBO J. 1991; 10(8):2023-31. PMC: 452883. DOI: 10.1002/j.1460-2075.1991.tb07732.x. View