Ultrastructural Immunohistochemical Localization of Vasopressin in the Hypothalamic-neurohypophysial System of Three Murids

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1976 Oct 22

PMID 791505

Citations 14

Authors

M Castel

J Hochman

Affiliations

Soon will be listed here.

Abstract

Vasopressin was immunohistochemically localized at the electron microscopic (EM) level in the hypothalamic-neurohypophysial system (HNS) of three murids. Antiserum to vasopressin was produced in rabbits injected with lysine vasopressin (LVP) conjugated to egg albumin (EA), anti-EA being precipitated prior to staining. Sternberger's unlabeled antibody peroxidase technique was employed, immunoreactivity being designated by peroxidase-anti-peroxidase (PAP) molecules and electron opacity. Immunoreactive neurosecretory granules (NSG) were found in the perikarya of the supraoptic nucleus (SON) in all three murids investigated, although far more profusely in the two wild strains. Immunoreactive axonal NSG were observed in the inner and outer zones of the median eminence (ME), and within most of the axons and terminals in the neurohypophysis. The concentration of primary serum effective for staining the SON (1:10-1:50) was far higher than that required for the ME and the neurohypophysis (1:500-1:1,200). Anti-LVP also induced electron opacity of granules in cells of the pars intermedia (PI). Discussion centers of the significance of immunoreactive NSG in the neurosecretory (NS) perikarya, on the possibility of an extragranular pool of hormone, and on speculation about the electron opacity of the PI granules.

Citing Articles

Neuropeptide and Small Transmitter Coexistence: Fundamental Studies and Relevance to Mental Illness.

Hokfelt T, Barde S, Xu Z, Kuteeva E, Ruegg J, Le Maitre E Front Neural Circuits. 2019; 12:106.

PMID: 30627087 PMC: 6309708. DOI: 10.3389/fncir.2018.00106.

Cellular and subcellular aquaporin-4 distribution in the mouse neurohypophysis and the effects of osmotic stimulation.

Mesbah-Benmessaoud O, Benabdesselam R, Hardin-Pouzet H, Dorbani-Mamine L, Grange-Messent V J Histochem Cytochem. 2011; 59(1):88-97.

PMID: 21339176 PMC: 3201125. DOI: 10.1369/jhc.2010.956805.

Nongranular vasopressin synthesis and transport in early stages of rehydration.

Krisch B Cell Tissue Res. 1980; 207(1):89-107.

PMID: 7388914 DOI: 10.1007/BF00239332.

Ultrastructure and immunocytochemistry of neurons in the supraoptic and paraventricular nuclei of the lizard Liolaemus cyanogaster. Evidence for the intracisternal location of the precursor of neurophysin.

Gonzalez C, Rodriguez E Cell Tissue Res. 1980; 207(3):463-77.

PMID: 6994886 DOI: 10.1007/BF00224620.

Ultrastructural localization of TRH-like immunoreactivity.

Johansson O, Hokfelt T, Jeffcoate S, White N, STERNBERGER L Exp Brain Res. 1980; 38(1):1-10.

PMID: 6766107 DOI: 10.1007/BF00237924.

References

Kordon C, Kerdelhue B, Pattou E, Jutisz M, SAWYER C . Immunocytochemical localization of LHRH in axons and nerve terminals of the rat median eminence. Proc Soc Exp Biol Med. 1974; 147(1):122-7. DOI: 10.3181/00379727-147-38294. View

Dreifuss J . A review on neurosecretory granules: their contents and mechanisms of release. Ann N Y Acad Sci. 1975; 248:184-201. DOI: 10.1111/j.1749-6632.1975.tb34185.x. View

Silverman A, Knigge K, Zimmerman E . Ultrastructural immunocytochemical localization of neurophysin in freeze-substituted neurohypophysis. Am J Anat. 1975; 142(2):265-71. DOI: 10.1002/aja.1001420209. View

VANDESANDE F, Demey J, Dierickx K . Identification of neurophysin producing cells. I. The origin of the neurophysin-like substance-containing nerve fibres of the external region of the median eminence of the rat. Cell Tissue Res. 1974; 151(2):187-200. DOI: 10.1007/BF00222222. View

Naik D . Influence of neurosecretion on the activity of median eminence and pars intermedia in hereditary nephrogenic diabetes insipidus mice with bilateral supraoptic lesions. Z Zellforsch Mikrosk Anat. 1972; 125(4):460-79. DOI: 10.1007/BF00306654. View

Zambrano D, De Robertis E . Ultrastructure of the hypothalamic neurosecretory system of the dog. Z Zellforsch Mikrosk Anat. 1967; 81(2):264-82. DOI: 10.1007/BF02075973. View

Castel M, Abraham M . Effects of a dry diet on the hypothalamic neurohypophyseal neurosecretory system in spiny mice as compared to the albino rat and mouse. Gen Comp Endocrinol. 1969; 12(2):231-41. DOI: 10.1016/0016-6480(69)90195-6. View

Belenky M, KONSTANTINOVA M, POLENOV A . On neurosecretory and adrenergic fibers in the intermediate lobe of the hypophysis in albino mice. Gen Comp Endocrinol. 1970; 15(2):185-97. DOI: 10.1016/0016-6480(70)90070-5. View

Naik D . Pituitary-adrenal relationships in mice with hereditary nephrogenic diabetes insipidus, with special emphasis on the neurohypophysis and pars intermedia. Z Zellforsch Mikrosk Anat. 1970; 107(3):317-42. DOI: 10.1007/BF00336671. View

10.

STERNBERGER L, PETRALI J . Quantitative immunocytochemistry of pituitary receptors for luteinizing hormone-releasing hormone. Cell Tissue Res. 1975; 162(2):141-76. DOI: 10.1007/BF00209204. View

11.

Sachs H, Takabatake Y . EVIDENCE FOR A PRECURSOR IN VASOPRESSIN BIOSYNTHESIS. Endocrinology. 1964; 75:943-8. DOI: 10.1210/endo-75-6-943. View

12.

Norstrom A . Release in vitro of neurohypophysial proteins from neural lobe tissue slices and from isolated neurosecretory granules of the rat. Z Zellforsch Mikrosk Anat. 1972; 129(1):114-39. DOI: 10.1007/BF00307114. View

13.

Moriarty G, HALMI N . Adrenocorticotropin production by the intermediate lobe of the rat pituitary. An electron microscopic-immunohistochemical study. Z Zellforsch Mikrosk Anat. 1972; 132(1):1-14. DOI: 10.1007/BF00310292. View

14.

Watkins W, Schwabedal P, Bock R . Immunohistochemical demonstration of a CRF-associated neurophysin in the external zone of the rat median eminence. Cell Tissue Res. 1974; 152(4):411-21. DOI: 10.1007/BF00218928. View

15.

Cannata M, Morris J . Changes in the appearance of hypothalamo-neurohypophysial neurosecretory granules associated with their maturation. J Endocrinol. 1973; 57(3):531-8. DOI: 10.1677/joe.0.0570531. View

16.

Moriarty G, Moriarty C, STERNBERGER L . Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J Histochem Cytochem. 1973; 21(9):825-33. DOI: 10.1177/21.9.825. View

17.

LECLERC R, Pelletier G . Electron microscope immunohistochemical localization of vasopressin in the hypothalamus and neurohypophysis of the normal and Brattleboro rat. Am J Anat. 1974; 140(4):583-8. DOI: 10.1002/aja.1001400410. View

18.

Zimmerman E, Carmel P, Husain M, Ferin M, TANNENBAUM M, FRANTZ A . Vasopressin and neurophysin: high concentrations in monkey hypophyseal portal blood. Science. 1973; 182(4115):925-7. DOI: 10.1126/science.182.4115.925. View

19.

Axen R, Porath J, Ernback S . Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967; 214(5095):1302-4. DOI: 10.1038/2141302a0. View

20.

Norstrom A . Subcellular distribution of neurophysin in rats subjected to haemorrhage, salt-loading and lactation and in rats with hereditary diabetes insipidus (Brattleboro strain). Z Zellforsch Mikrosk Anat. 1973; 140(3):413-24. DOI: 10.1007/BF00307027. View