Localization of Neurophysin Within Organelles Associated with Protein Synthesis and Packaging in the Hypothalamoneurohypophysial System: an Immunocytochemical Study

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Nov 1

PMID 392513

Citations 9

Authors

R D Broadwell

C Oliver

M W Brightman

Affiliations

Soon will be listed here.

Abstract

Electron microscopic immunocytochemical localization of neurophysin in the hypothalamo-neurohypophysial system of mice has been studied by using the pre-embedding staining approach to the unlabeled antibody-enzyme technique of Sternberger. In supraoptic cell bodies, peroxidase-antiperoxidase reaction product was localized within cisternae of the nuclear envelope, rough endoplasmic reticulum, and Golgi saccules but not in GERL (Golgi-associated smooth endoplasmic reticulum from which lysosomes arise). Reaction product was also present in secondary lysosomes. Secretory granules in supraoptic perikarya and posterior pituitary Herring bodies were likewise immunoreactive. These findings with the unlabeled antibody-enzyme technique provide conclusive evidence for the localization of an antigen within cellular organelles associated with protein synthesis and packaging.

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References

WELLER T, Coons A . Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954; 86(4):789-94. DOI: 10.3181/00379727-86-21235. View

Halasz N, Ljungdahl A, Hokfelt T, Johansson O, Goldstein M, Park D . Transmitter histochemistry of the rat olfactory bulb. I. Immunohistochemical localization of monoamine synthesizing enzymes. Support for intrabulbar, periglomerular dopamine neurons. Brain Res. 1977; 126(3):455-74. DOI: 10.1016/0006-8993(77)90597-2. View

Pickel V, Joh T, Reis D, Leeman S, Miller R . Electron microscopic localization of substance P and enkephalin in axon terminals related to dendrites of catecholaminergic neurons. Brain Res. 1979; 160(3):387-400. DOI: 10.1016/0006-8993(79)91068-0. View

Sofroniew M, Weindl A, Schinko I, WETZSTEIN R . The distribution of vasopressin-, oxytocin-, and neurophysin-producing neurons in the guinea pig brain. I. The classical hypothalamo-neurophypophyseal system. Cell Tissue Res. 1979; 196(3):367-84. DOI: 10.1007/BF00234734. View

Barber R, Vaughn J, Slemmon J, Salvaterra P, Roberts E, Leeman S . The origin, distribution and synaptic relationships of substance P axons in rat spinal cord. J Comp Neurol. 1979; 184(2):331-51. DOI: 10.1002/cne.901840208. View

Sar M, Stumpf W, Miller R, Chang K, Cuatrecasas P . Immunohistochemical localization of enkephalin in rat brain and spinal cord. J Comp Neurol. 1978; 182(1):17-37. DOI: 10.1002/cne.901820103. View

Sofroniew M, Weindl A . Projections from the parvocellular vasopressin- and neurophysin-containing neurons of the suprachiasmatic nucleus. Am J Anat. 1978; 153(3):391-429. DOI: 10.1002/aja.1001530305. View

Kozlowski G, FRENK S, Brownfield M . Localization of neurophysin in the rat supraoptic nucleus. I. Ultrastructural immunocytochemistry using the post-embedding technique. Cell Tissue Res. 1977; 179(4):467-73. DOI: 10.1007/BF00219849. View

van Leeuwen F . Immunoelectron microscopic visualization of neurohypophyseal hormones: evaluation of some tissue preparations and staining procedures. J Histochem Cytochem. 1977; 25(11):1213-21. DOI: 10.1177/25.11.334961. View

10.

Chan-Palay V, PALAY S . Ultrastructural identification of substance P cells and their processes in rat sensory ganglia and their terminals in the spinal cord by immunocytochemistry. Proc Natl Acad Sci U S A. 1977; 74(9):4050-4. PMC: 431840. DOI: 10.1073/pnas.74.9.4050. View

11.

van Leeuwen F, Swaab D . Specific immunoelectronmicroscopic localization of vasopressin and oxytocin in the neurohypophysis of the rat. Cell Tissue Res. 1977; 177(4):493-501. DOI: 10.1007/BF00220610. View

12.

Kraehenbuhl J, Racine L, JAMIESON J . Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells. J Cell Biol. 1977; 72(2):406-23. PMC: 2111013. DOI: 10.1083/jcb.72.2.406. View

13.

van Leeuwen F, Swaab D, de Raay C . Immunoelectronmicroscopic localization of vasopressin in the rat suprachiasmatic nucleus. Cell Tissue Res. 1978; 193(1):1-10. DOI: 10.1007/BF00221596. View

14.

Buijs R . Intra- and extrahypothalamic vasopressin and oxytocin pathways in the rat. Pathways to the limbic system, medulla oblongata and spinal cord. Cell Tissue Res. 1978; 192(3):423-35. DOI: 10.1007/BF00212323. View

15.

Barber R, Vaughn J, Saito K, McLaughlin B, Roberts E . GABAergic terminals are presynaptic to primary afferent terminals in the substantia gelatinosa of the rat spinal cord. Brain Res. 1978; 141(1):35-55. DOI: 10.1016/0006-8993(78)90615-7. View

16.

Cuello A, Jessell T, Kanazawa I, Iversen L . Substance P: localization in synaptic vesicles in rat central nervous system. J Neurochem. 1977; 29(4):747-51. DOI: 10.1111/j.1471-4159.1977.tb07795.x. View

17.

Hand A, Oliver C . Relationship between the Golgi apparatus, GERL, and secretory granules in acinar cells of the rat exorbital lacrimal gland. J Cell Biol. 1977; 74(2):399-413. PMC: 2110078. DOI: 10.1083/jcb.74.2.399. View

18.

Gainer H, Sarne Y, Brownstein M . Biosynthesis and axonal transport of rat neurohypophysial proteins and peptides. J Cell Biol. 1977; 73(2):366-81. PMC: 2109920. DOI: 10.1083/jcb.73.2.366. View

19.

Ribak C, Vaughn J, Saito K, Barber R, Roberts E . Glutamate decarboxylase localization in neurons of the olfactory bulb. Brain Res. 1977; 126(1):1-18. DOI: 10.1016/0006-8993(77)90211-6. View

20.

Pickel V, Reis D, Leeman S . Ultrastructural localization of substance P in neurons of rat spinal cord. Brain Res. 1977; 122(3):534-40. DOI: 10.1016/0006-8993(77)90463-2. View