Intestinal Expression and Cellular Immune Responses to Human Heat-shock Protein 60 in Crohn's Disease

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 1994 Mar 1

PMID 7907543

Citations 4

Authors

M E Baca-Estrada

R S Gupta

R H Stead

K Croitoru

Affiliations

Soon will be listed here.

Abstract

Changes in the intestinal expression of the endogenous human 60-kDa heat-shock protein (HSP60) were investigated in patients with Crohn's disease. HSP60 immunoreactivity was detected in epithelial cells, vascular smooth muscle, and nerve cell bodies of both small and large bowel from patients with Crohn's disease. However, control tissue showed a similar pattern of HSP60 expression. Western blot analysis confirmed that the HSP60 immunoreactivity detected in the intestine corresponded to the 60-kDa HSP. The proliferative response of peripheral blood lymphocytes (PBL) and intestinal intraepithelial lymphocytes (IEL) to recombinant human HSP60 was examined. The results indicate that there was no significant difference in responses between patients with Crohn's disease and controls. Furthermore, there was no increase in the proportion of gamma/delta T cell receptor-bearing T cells in PBL from patients with Crohn's disease cultured for six days in the presence of human HSP60 as compared to control patients. These results suggest that endogenous human HSP60 is unlikely to be a target for an autoimmune response in patients with Crohn's disease.

Citing Articles

Chronic heat stress regulates the relation between heat shock protein and immunity in broiler small intestine.

Siddiqui S, Kang D, Park J, Khan M, Shim K Sci Rep. 2020; 10(1):18872.

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Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis.

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Impaired humoral immune response against mycobacterial 65-kDa heat shock protein (HSP65) in patients with inflammatory bowel disease.

Bene L, Fust G, Huszti Z, Hernadi Z, Fekete B, Meszaros M Dig Dis Sci. 2002; 47(7):1432-7.

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Intestinal expression of human heat shock protein 90 in patients with Crohn's disease and ulcerative colitis.

Stahl M, Ludwig D, Fellermann K, Stange E Dig Dis Sci. 1998; 43(5):1079-87.

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References

THAYER Jr W, Coutu J, Chiodini R, Van Kruiningen H, MERKAL R . Possible role of mycobacteria in inflammatory bowel disease. II. Mycobacterial antibodies in Crohn's disease. Dig Dis Sci. 1984; 29(12):1080-5. DOI: 10.1007/BF01317079. View

Cohen I, Young D . Autoimmunity, microbial immunity and the immunological homunculus. Immunol Today. 1991; 12(4):105-10. DOI: 10.1016/0167-5699(91)90093-9. View

Chiodini R . Crohn's disease and the mycobacterioses: a review and comparison of two disease entities. Clin Microbiol Rev. 1989; 2(1):90-117. PMC: 358101. DOI: 10.1128/CMR.2.1.90. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Graham D, Markesich D, Yoshimura H . Mycobacteria and inflammatory bowel disease. Results of culture. Gastroenterology. 1987; 92(2):436-42. DOI: 10.1016/0016-5085(87)90139-9. View

Yoshimura H, Graham D, Estes M, MERKAL R . Investigation of association of mycobacteria with inflammatory bowel disease by nucleic acid hybridization. J Clin Microbiol. 1987; 25(1):45-51. PMC: 265821. DOI: 10.1128/jcm.25.1.45-51.1987. View

Cho S, Brennan P, Yoshimura H, Korelitz B, Graham D . Mycobacterial aetiology of Crohn's disease: serologic study using common mycobacterial antigens and a species-specific glycolipid antigen from Mycobacterium paratuberculosis. Gut. 1986; 27(11):1353-6. PMC: 1434076. DOI: 10.1136/gut.27.11.1353. View

Balk S, Ebert E, Blumenthal R, McDermott F, Wucherpfennig K, Landau S . Oligoclonal expansion and CD1 recognition by human intestinal intraepithelial lymphocytes. Science. 1991; 253(5026):1411-5. DOI: 10.1126/science.1716785. View

Gaston J, Life P, Bailey L, Bacon P . In vitro responses to a 65-kilodalton mycobacterial protein by synovial T cells from inflammatory arthritis patients. J Immunol. 1989; 143(8):2494-500. View

10.

Kaufmann S, Kabelitz D . Gamma/delta T lymphocytes and heat shock proteins. Curr Top Microbiol Immunol. 1991; 167:191-207. DOI: 10.1007/978-3-642-75875-1_11. View

11.

van Eden W, Thole J, van der Zee R, Noordzij A, van Embden J, Hensen E . Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis. Nature. 1988; 331(6152):171-3. DOI: 10.1038/331171a0. View

12.

Cohen I . A heat shock protein, molecular mimicry and autoimmunity. Isr J Med Sci. 1990; 26(12):673-6. View

13.

Okada C, Holzmann B, Guidos C, Palmer E, Weissman I . Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990; 144(9):3473-7. View

14.

Shoenfeld Y, Isenberg D . Mycobacteria and autoimmunity. Immunol Today. 1988; 9(6):178-82. DOI: 10.1016/0167-5699(88)91294-7. View

15.

Nagler-Anderson C, McNair L, Cradock A . Self-reactive, T cell receptor-gamma delta+, lymphocytes from the intestinal epithelium of weanling mice. J Immunol. 1992; 149(7):2315-22. View

16.

Healy A, Mariethoz E, Pizurki L, Polla B . Heat shock proteins in cellular defense mechanisms and immunity. Ann N Y Acad Sci. 1992; 663:319-30. DOI: 10.1111/j.1749-6632.1992.tb38675.x. View

17.

Van Kruiningen H, Chiodini R, Thayer W, Coutu J, MERKAL R, Runnels P . Experimental disease in infant goats induced by a Mycobacterium isolated from a patient with Crohn's disease. A preliminary report. Dig Dis Sci. 1986; 31(12):1351-60. DOI: 10.1007/BF01299814. View

18.

Jindal S, Dudani A, Singh B, Harley C, Gupta R . Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen. Mol Cell Biol. 1989; 9(5):2279-83. PMC: 363030. DOI: 10.1128/mcb.9.5.2279-2283.1989. View

19.

Holoshitz J, Koning F, Coligan J, de Bruyn J, Strober S . Isolation of CD4- CD8- mycobacteria-reactive T lymphocyte clones from rheumatoid arthritis synovial fluid. Nature. 1989; 339(6221):226-9. DOI: 10.1038/339226a0. View

20.

Stainsby K, Lowes J, Allan R, Ibbotson J . Antibodies to Mycobacterium paratuberculosis and nine species of environmental mycobacteria in Crohn's disease and control subjects. Gut. 1993; 34(3):371-4. PMC: 1374144. DOI: 10.1136/gut.34.3.371. View