Mycobacterial Aetiology of Crohn's Disease: Serologic Study Using Common Mycobacterial Antigens and a Species-specific Glycolipid Antigen from Mycobacterium Paratuberculosis

Overview

Journal Gut

Specialty Gastroenterology

Date 1986 Nov 1

PMID 3792917

Citations 16

Authors

S N Cho

P J Brennan

H H Yoshimura

B I Korelitz

D Y Graham

Affiliations

Soon will be listed here.

Abstract

Crohn's disease is a granulomatous form of enteritis superficially similar to Johne's disease (paratuberculosis) of ruminants. Recently, a Mycobacterium sp closely related to Mycobacterium paratuberculosis was cultured from tissues of patients with Crohn's disease suggesting that M paratuberculosis may be the aetiologic agent in some cases. In addition, greater seroreactivity to M paratuberculosis has been reported in patients with Crohn's disease. In the present study, we have evaluated the serum antibody response to disrupted M paratuberculosis using ELISA and serum specimens from 33 people with Crohn's disease, 21 with ulcerative colitis, and 12 non-inflammatory bowel disease controls. We failed to find a consistent IgG, IgM, or IgA antibody response to Mycobacterium paratuberculosis. The results indicate that, as in bovine paratuberculosis, serum seroreactivity is not a reliable tool for examining the relationship between human intestinal disease and mycobacteria.

Citing Articles

Isotype-specific Antibody Responses to Mycobacterium avium paratuberculosis Antigens Are Associated With the Use of Biologic Therapy in Inflammatory Bowel Disease.

van der Sloot K, Voskuil M, Blokzijl T, Dinkla A, Ravesloot L, Visschedijk M J Crohns Colitis. 2020; 15(8):1253-1263.

PMID: 33378524 PMC: 8496491. DOI: 10.1093/ecco-jcc/jjaa263.

Nucleotide sequence analysis and seroreactivities of the 65K heat shock protein from Mycobacterium paratuberculosis.

Naser S, Engstrand L, Burch P, Hachem C, Whipple D, Graham D Clin Diagn Lab Immunol. 1995; 2(6):657-64.

PMID: 8574825 PMC: 170216. DOI: 10.1128/cdli.2.6.657-664.1995.

Antibodies to Mycobacterium paratuberculosis and nine species of environmental mycobacteria in Crohn's disease and control subjects.

Stainsby K, Lowes J, Allan R, Ibbotson J Gut. 1993; 34(3):371-4.

PMID: 8472986 PMC: 1374144. DOI: 10.1136/gut.34.3.371.

Paratuberculosis.

Cocito C, Gilot P, Coene M, De Kesel M, Poupart P, Vannuffel P Clin Microbiol Rev. 1994; 7(3):328-45.

PMID: 7923053 PMC: 358329. DOI: 10.1128/CMR.7.3.328.

Intestinal expression and cellular immune responses to human heat-shock protein 60 in Crohn's disease.

Baca-Estrada M, Gupta R, Stead R, Croitoru K Dig Dis Sci. 1994; 39(3):498-506.

PMID: 7907543 DOI: 10.1007/BF02088334.

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