Heat Shock Protein 70 Overexpression Affects the Response to Ultraviolet Light in Murine Fibroblasts. Evidence for Increased Cell Viability and Suppression of Cytokine Release

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1995 Mar 1

PMID 7883992

Citations 49

Authors

M M Simon

A Reikerstorfer

A Schwarz

C Krone

T A Luger

M Jaattela

T Schwarz

Affiliations

Soon will be listed here.

Abstract

To elucidate cellular concepts for protection against ultraviolet (UV) light we investigated the effect of heat shock protein 70 (hsp70) overexpression on cell viability and on the secretion of UV-inducible immunological cytokines. Transfected murine fibrosarcoma cells (WEHI-S), overexpressing hsp70 or a sham transfected control were used. Overexpression of hsp70 was sufficient to markedly increase cell viability upon treatment with UVB (290-320 nm). Since long wave UV (UVA, 320-400 nm) as well as UVB turned out to stimulate the release of O2- radicals we studied the cell viability upon oxidative stress. Hsp70 overexpression increased viability upon treatment with hydrogen peroxide or menadione, but had no influence on UV-induced O2- release. UV-light is known to upregulate immunologic and proinflammatory cytokines such as IL-1 and IL-6. Oxidative stress appeared to exert a similar effect. Hsp70 overexpression markedly decreased the release of IL-6 induced by UVA, UVB and oxidative stress. To test whether the hsp70 mediated suppression is confined to events caused by UV-light we determined IL-1-mediated effects. IL-1-induced IL-6 release was reduced by hsp70 overexpression, whereas the IL-1 mediated activation of nuclear factor kappa B was not affected. Our data suggests that hsp70 plays a central role not only in cell protection against UV-light, but also in the regulation of proinflammatory cytokine release induced by UV-exposure.

Citing Articles

Mesenchymal Stem Cell Behavior under Microgravity: From Stress Response to a Premature Senescence.

Pala R, Cruciani S, Manca A, Garroni G, El Faqir M, Lentini V Int J Mol Sci. 2023; 24(9).

PMID: 37175460 PMC: 10178040. DOI: 10.3390/ijms24097753.

Protective Effects of Fermented Houttuynia cordata Against UVA and HO-Induced Oxidative Stress in Human Skin Keratinocytes.

Kwon N, Vinayagam R, Do G, Lee K, Kang S Appl Biochem Biotechnol. 2022; 195(5):3027-3046.

PMID: 36495375 DOI: 10.1007/s12010-022-04241-8.

Melatonin: Regulation of Prion Protein Phase Separation in Cancer Multidrug Resistance.

Loh D, Reiter R Molecules. 2022; 27(3).

PMID: 35163973 PMC: 8839844. DOI: 10.3390/molecules27030705.

Heat Shock Proteins and Their Role in Pregnancy: Redefining the Function of "Old Rum in a New Bottle".

Jee B, Dhar R, Singh S, Karmakar S Front Cell Dev Biol. 2021; 9:648463.

PMID: 33996811 PMC: 8116900. DOI: 10.3389/fcell.2021.648463.

Molecular characterization and expression patterns of heat shock proteins in Spodoptera littoralis, heat shock or immune response?.

Guz N, Dageri A, Altincicek B, Aksoy S Cell Stress Chaperones. 2020; 26(1):29-40.

PMID: 32803739 PMC: 7736435. DOI: 10.1007/s12192-020-01149-2.

References

Kock A, Schwarz T, Kirnbauer R, Urbanski A, Perry P, Ansel J . Human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. J Exp Med. 1990; 172(6):1609-14. PMC: 2188768. DOI: 10.1084/jem.172.6.1609. View

Gromkowski S, Yagi J, Janeway Jr C . Elevated temperature regulates tumor necrosis factor-mediated immune killing. Eur J Immunol. 1989; 19(9):1709-14. DOI: 10.1002/eji.1830190927. View

Aida Y, Pabst M . Neutrophil responses to lipopolysaccharide. Effect of adherence on triggering and priming of the respiratory burst. J Immunol. 1991; 146(4):1271-6. View

Shimizu H, Mitomo K, Watanabe T, Okamoto S, Yamamoto K . Involvement of a NF-kappa B-like transcription factor in the activation of the interleukin-6 gene by inflammatory lymphokines. Mol Cell Biol. 1990; 10(2):561-8. PMC: 360838. DOI: 10.1128/mcb.10.2.561-568.1990. View

Nutter L, Ngo E, FISHER G, Gutierrez P . DNA strand scission and free radical production in menadione-treated cells. Correlation with cytotoxicity and role of NADPH quinone acceptor oxidoreductase. J Biol Chem. 1992; 267(4):2474-9. View

Hopkins S, Humphreys M . Simple, sensitive and specific bioassay of interleukin-1. J Immunol Methods. 1989; 120(2):271-6. DOI: 10.1016/0022-1759(89)90252-4. View

Ferris D, Harel-Bellan A, Morimoto R, Welch W, Farrar W . Mitogen and lymphokine stimulation of heat shock proteins in T lymphocytes. Proc Natl Acad Sci U S A. 1988; 85(11):3850-4. PMC: 280317. DOI: 10.1073/pnas.85.11.3850. View

Black H . Potential involvement of free radical reactions in ultraviolet light-mediated cutaneous damage. Photochem Photobiol. 1987; 46(2):213-21. DOI: 10.1111/j.1751-1097.1987.tb04759.x. View

Vitelli L, Kemler I, Lauber B, Birnstiel M, Busslinger M . Developmental regulation of micro-injected histone genes in sea urchin embryos. Dev Biol. 1988; 127(1):54-63. DOI: 10.1016/0012-1606(88)90188-1. View

10.

Lindquist S, Craig E . The heat-shock proteins. Annu Rev Genet. 1988; 22:631-77. DOI: 10.1146/annurev.ge.22.120188.003215. View

11.

Israel A, Le Bail O, Hatat D, Piette J, Kieran M, Logeat F . TNF stimulates expression of mouse MHC class I genes by inducing an NF kappa B-like enhancer binding activity which displaces constitutive factors. EMBO J. 1989; 8(12):3793-800. PMC: 402065. DOI: 10.1002/j.1460-2075.1989.tb08556.x. View

12.

Riabowol K, Mizzen L, Welch W . Heat shock is lethal to fibroblasts microinjected with antibodies against hsp70. Science. 1988; 242(4877):433-6. DOI: 10.1126/science.3175665. View

13.

Jaattela M, Wissing D, Bauer P, Li G . Major heat shock protein hsp70 protects tumor cells from tumor necrosis factor cytotoxicity. EMBO J. 1992; 11(10):3507-12. PMC: 556808. DOI: 10.1002/j.1460-2075.1992.tb05433.x. View

14.

Simon M, Aragane Y, Schwarz A, Luger T, Schwarz T . UVB light induces nuclear factor kappa B (NF kappa B) activity independently from chromosomal DNA damage in cell-free cytosolic extracts. J Invest Dermatol. 1994; 102(4):422-7. DOI: 10.1111/1523-1747.ep12372194. View

15.

Baeuerle P . The inducible transcription activator NF-kappa B: regulation by distinct protein subunits. Biochim Biophys Acta. 1991; 1072(1):63-80. DOI: 10.1016/0304-419x(91)90007-8. View

16.

Sen R, Baltimore D . Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell. 1986; 46(5):705-16. DOI: 10.1016/0092-8674(86)90346-6. View

17.

Jaattela M . Overexpression of major heat shock protein hsp70 inhibits tumor necrosis factor-induced activation of phospholipase A2. J Immunol. 1993; 151(8):4286-94. View

18.

Jaattela M, Saksela K, Saksela E . Heat shock protects WEHI-164 target cells from the cytolysis by tumor necrosis factors alpha and beta. Eur J Immunol. 1989; 19(8):1413-7. DOI: 10.1002/eji.1830190810. View

19.

Braselmann S, Graninger P, Busslinger M . A selective transcriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins. Proc Natl Acad Sci U S A. 1993; 90(5):1657-61. PMC: 45938. DOI: 10.1073/pnas.90.5.1657. View

20.

Urbanski A, Schwarz T, Neuner P, Krutmann J, Kirnbauer R, Kock A . Ultraviolet light induces increased circulating interleukin-6 in humans. J Invest Dermatol. 1990; 94(6):808-11. DOI: 10.1111/1523-1747.ep12874666. View