Expression of Genomic and CDNA Transgenes After Co-integration in Transgenic Mice

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 1995 Jan 1

PMID 7881461

Citations 8

Authors

R A McKnight

R J Wall

L Hennighausen

Affiliations

Soon will be listed here.

Abstract

In general, genomic transgenes are expressed efficiently in mice, while their cDNA-based transgenes are frequently silent. Clark et al. (1992) have shown that silent cDNA transgenes under the control of the sheep beta-lactoglobulin promoter can be activated after co-injecting them with a genomic sheep beta-lactoglobulin transgene. We have tested the general utility of this concept using mouse whey acidic protein (WAP) transgenes. Here we show that WAP cDNA transgenes are virtually silent in transgenic mice. In contrast, WAP transgenes containing all introns are expressed in approximately 50% of the lines at levels ranging from 1% to more than 100% of the endogenous RNA (McKnight et al., 1992). When a WAP-genomic transgene was co-injected with a WAP-cDNA, basal activation of the cDNA was possible. However, the activity of the WAP-cDNA transgene did not exceed 1% of the WAP-genomic transgene. This suggests that a permissive integration site capable of supporting basal level transcription can be established, but further events are required for full activation of the transgene.

Citing Articles

Hierarchy within the mammary STAT5-driven Wap super-enhancer.

Shin H, Willi M, HyunYoo K, Zeng X, Wang C, Metser G Nat Genet. 2016; 48(8):904-911.

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Making better transgenic models: conditional, temporal, and spatial approaches.

Ristevski S Mol Biotechnol. 2005; 29(2):153-63.

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Secretion of recombinant human fibrinogen by the murine mammary gland.

Butler S, OSickey T, Lord S, Lubon H, Gwazdauskas F, Velander W Transgenic Res. 2004; 13(5):437-50.

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Rescue of an MMTV transgene by co-integration reveals novel locus control properties of the ovine beta-lactoglobulin gene that confer locus commitment to heterogeneous tissues.

Langley B, Vilotte J, Stinnakre M, Whitelaw C, LHuillier P Transgenic Res. 1999; 7(3):205-12.

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Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant human protein C.

Van Cott K, Lubon H, Russell C, Butler S, Gwazdauskas F, Knight J Transgenic Res. 1997; 6(3):203-12.

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