Virion Incorporation of Envelope Glycoproteins with Long but Not Short Cytoplasmic Tails is Blocked by Specific, Single Amino Acid Substitutions in the Human Immunodeficiency Virus Type 1 Matrix

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1995 Mar 1

PMID 7853546

Citations 202

Authors

E O Freed

M A Martin

Affiliations

Soon will be listed here.

Abstract

Incorporation of envelope glycoproteins into a budding retrovirus is an essential step in the formation of an infectious virus particle. By using site-directed mutagenesis, we identified specific amino acid residues in the matrix domain of the human immunodeficiency virus type 1 (HIV-1) Gag protein that are critical to the incorporation of HIV-1 envelope glycoproteins into virus particles. Pseudotyping analyses were used to demonstrate that two heterologous envelope glycoproteins with short cytoplasmic tails (the envelope of the amphotropic murine leukemia virus and a naturally truncated HIV-2 envelope) are efficiently incorporated into HIV-1 particles bearing the matrix mutations. Furthermore, deletion of the cytoplasmic tail of HIV-1 transmembrane envelope glycoprotein gp41 from 150 to 7 or 47 residues reversed the incorporation block imposed by the matrix mutations. These results suggest the existence of a specific functional interaction between the HIV-1 matrix and the gp41 cytoplasmic tail.

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