Reverse Transcription-PCR Detection of LaCrosse Virus in Mosquitoes and Comparison with Enzyme Immunoassay and Virus Isolation

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1994 Sep 1

PMID 7814527

Citations 7

Authors

L P Wasieloski Jr

L A Curtis

C D Blair

B J Beaty

Affiliations

Soon will be listed here.

Abstract

A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools. All three techniques were able to detect a single LAC-infected mosquito in a pool of 99 negative mosquitoes. Virus isolation was the most sensitive of the three techniques; it was possible to isolate virus immediately following intrathoracic inoculation of mosquitoes. RT-PCR was second in sensitivity; LAC RNA was detected 1 day postinfection. EIA detected LAC antigen 2 days postinfection. Additionally, RT-PCR and EIA were able to detect LAC RNA and protein, respectively, from mosquito samples which were subjected to seven freeze-thaw cycles, and RT-PCR was able to detect LAC RNA from mosquito samples which remained at room temperature for up to 7 days.

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Nucleic acid amplification assays for detection of La Crosse virus RNA.

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Characterization of La Crosse virus RNA in autopsied central nervous system tissues.

Chandler L, Borucki M, Dobie D, Wasieloski L, Thompson W, Gundersen C J Clin Microbiol. 1998; 36(11):3332-6.

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Analysis of LaCrosse virus S mRNA 5' termini in infected mosquito cells and Aedes triseriatus mosquitoes.

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PMID: 9151829 PMC: 191657. DOI: 10.1128/JVI.71.6.4395-4399.1997.

Analysis of La Crosse virus S-segment RNA and its positive-sense transcripts in persistently infected mosquito tissues.

Chandler L, Wasieloski L, Blair C, Beaty B J Virol. 1996; 70(12):8972-6.

PMID: 8971026 PMC: 190994. DOI: 10.1128/JVI.70.12.8972-8976.1996.

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