Demonstration of HCV-RNA and HBV-DNA in the Serum of HBsAg Negative Patients with Hepatocellular Carcinoma

Overview

Journal Eur J Epidemiol

Specialty Public Health

Date 1994 Apr 1

PMID 7813697

Authors

J Enriquez

K Fuchs

F J Martinez Cerezo

R Seminago

P Madoz

J Torras

M Roggendorf

Affiliations

Soon will be listed here.

Abstract

Forty patients with chronic liver disease and HCC were analyzed for infection with hepatitis C (HCV) and hepatitis B (HBV) viruses. All patients were negative for HBsAg, 16 were alcoholics, 6 had previous blood transfusions and 18 had sporadic chronic hepatitis. Antibodies to HCV were determined by EIA 2nd generation. HBV-DNA was detected by PCR using primers of the precore region. Analysis of HCV-RNA was done with nested PCR amplifying the 5' non-coding region of the HCV genome, using primers complementary to nucleotides 1-20 and 305-320 and nested primers complementary to nucleotides 21-31 and 271-286 of the HCV-J1. Anti-HCV were positive in 35/40 patients (87.5%). HCV-RNA was detected by PCR in 34 patients (85%) all of them positive for the anti-HCV. HCV-RNA was detected in 70.5% of the alcohol abusers, in 100% of patients with history of transfusion(s) and 94.1% of patients with cryptogenic chronic liver disease. HBV-DNA was detected in only 2 patients. In conclusion, there is a high rate of HCV and a low rate of HBV viremia detected by PCR in Spanish patients with HCC HBsAg negative. No patient without anti-HCV presents HCV-RNA. Our results suggest that persistent HCV replication may play a role in hepatic carcinogenesis, as HBV-DNA could be found in only 5% of our HCC patients.

References

Hasan F, Jeffers L, de Medina M, Reddy K, Parker T, Schiff E . Hepatitis C-associated hepatocellular carcinoma. Hepatology. 1990; 12(3 Pt 1):589-91. DOI: 10.1002/hep.1840120323. View

Hsu T, Fourel G, Etiemble J, Tiollais P, Buendia M . Integration of hepatitis virus DNA near c-myc in woodchuck hepatocellular carcinoma. Gastroenterol Jpn. 1990; 25 Suppl 2:43-8. DOI: 10.1007/BF02779927. View

Bruix J, Barrera J, Calvet X, Ercilla G, Costa J, Sanchez-Tapias J . Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis. Lancet. 1989; 2(8670):1004-6. DOI: 10.1016/s0140-6736(89)91015-5. View

Farinati F, Fagiuoli S, de Maria N, Chiaramonte M, Aneloni V, Ongaro S . Anti-HCV positive hepatocellular carcinoma in cirrhosis. Prevalence, risk factors and clinical features. J Hepatol. 1992; 14(2-3):183-7. DOI: 10.1016/0168-8278(92)90156-j. View

Lai M, Chen P, Yang P, Sheu J, SUNG J, Chen D . Identification and characterization of intrahepatic hepatitis B virus DNA in HBsAg-seronegative patients with chronic liver disease and hepatocellular carcinoma in Taiwan. Hepatology. 1990; 12(3 Pt 1):575-81. DOI: 10.1002/hep.1840120321. View

Kiyosawa K, Sodeyama T, Tanaka E, Gibo Y, Yoshizawa K, Nakano Y . Interrelationship of blood transfusion, non-A, non-B hepatitis and hepatocellular carcinoma: analysis by detection of antibody to hepatitis C virus. Hepatology. 1990; 12(4 Pt 1):671-5. DOI: 10.1002/hep.1840120409. View

Takada S, Gotoh Y, Hayashi S, Kobayashi M, Koike K . Integrated structures of HBV DNA in chronic hepatitis and hepatoma tissues. Gastroenterol Jpn. 1990; 25 Suppl 2:31-7. DOI: 10.1007/BF02779925. View

Ruiz J, Sangro B, Cuende J, Beloqui O, Riezu-Boj J, Herrero J . Hepatitis B and C viral infections in patients with hepatocellular carcinoma. Hepatology. 1992; 16(3):637-41. DOI: 10.1002/hep.1840160305. View

Zaaijer H, Cuypers H, Reesink H, Winkel I, Gerken G, Lelie P . Reliability of polymerase chain reaction for detection of hepatitis C virus. Lancet. 1993; 341(8847):722-4. DOI: 10.1016/0140-6736(93)90488-3. View

10.

Okamoto H, Okada S, Sugiyama Y, Yotsumoto S, Tanaka T, Yoshizawa H . The 5'-terminal sequence of the hepatitis C virus genome. Jpn J Exp Med. 1990; 60(3):167-77. View

11.

Brillanti S, Barbara L, Miglioli M, Bonino F . Hepatitis C virus: a possible cause of chronic hepatitis in alcoholics. Lancet. 1989; 2(8676):1390-1. DOI: 10.1016/s0140-6736(89)91992-2. View

12.

Nishioka K, Watanabe J, Furuta S, Tanaka E, Iino S, Suzuki H . A high prevalence of antibody to the hepatitis C virus in patients with hepatocellular carcinoma in Japan. Cancer. 1991; 67(2):429-33. DOI: 10.1002/1097-0142(19910115)67:2<429::aid-cncr2820670218>3.0.co;2-#. View

13.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

14.

Fourel G, Trepo C, Bougueleret L, Henglein B, Ponzetto A, Tiollais P . Frequent activation of N-myc genes by hepadnavirus insertion in woodchuck liver tumours. Nature. 1990; 347(6290):294-8. DOI: 10.1038/347294a0. View

15.

Choo Q, Kuo G, Weiner A, Overby L, Bradley D, Houghton M . Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989; 244(4902):359-62. DOI: 10.1126/science.2523562. View

16.

Sheu J, Huang G, Shih L, Lee W, Chou H, Wang J . Hepatitis C and B viruses in hepatitis B surface antigen-negative hepatocellular carcinoma. Gastroenterology. 1992; 103(4):1322-7. DOI: 10.1016/0016-5085(92)91523-7. View

17.

Cristiano K, Di Bisceglie A, Hoofnagle J, Feinstone S . Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: detection by the polymerase chain reaction using multiple primer sets. Hepatology. 1991; 14(1):51-5. DOI: 10.1002/hep.1840140109. View

18.

Ohkoshi S, Kojima H, Tawaraya H, Miyajima T, Kamimura T, Asakura H . Prevalence of antibody against non-A, non-B hepatitis virus in Japanese patients with hepatocellular carcinoma. Jpn J Cancer Res. 1990; 81(6-7):550-3. PMC: 6504047. DOI: 10.1111/j.1349-7006.1990.tb02605.x. View

19.

Fuchs K, Motz M, Schreier E, Zachoval R, Deinhardt F, Roggendorf M . Characterization of nucleotide sequences from European hepatitis C virus isolates. Gene. 1991; 103(2):163-9. DOI: 10.1016/0378-1119(91)90269-h. View

20.

Colombo M, Kuo G, Choo Q, Donato M, Del Ninno E, Tommasini M . Prevalence of antibodies to hepatitis C virus in Italian patients with hepatocellular carcinoma. Lancet. 1989; 2(8670):1006-8. DOI: 10.1016/s0140-6736(89)91016-7. View