The Processing of Macronuclear-destined DNA Sequences Microinjected into the Macronuclear Anlagen of the Hypotrichous Ciliate Stylonchia Lemnae

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1995 May 25

PMID 7784174

Citations 3

Authors

J P Wen

C Eder

H J Lipps

Affiliations

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Abstract

We describe the construction of a vector carrying the micronuclear versions of two macronuclear DNA molecules, one of which was modified by the insertion of a polylinker sequence. This vector was injected into the polytene chromosomes of the developing macronucleus of Stylonychia and its processing during further macronuclear development and its fate in the mature macronucleus were analyzed. In up to 30% of injected cells the modified macronuclear DNA sequence could be detected. While the internal eliminated sequences (IES) present in the macronuclear precursor DNA sequence are still retained in the mature macronucleus, the modified macronuclear DNA sequence is correctly cut out from the vector, telomeres are added de novo and it is stably retained in the macronucleus during vegetative growth of the cells. This vector system represents an experimental system that allows the identification of DNA sequences involved in the processing of macronuclear DNA sequences during macronuclear development.

Citing Articles

Both subtelomeric regions are required and sufficient for specific DNA fragmentation during macronuclear development in Stylonychia lemnae.

Jonsson F, Steinbruck G, Lipps H Genome Biol. 2001; 2(2):RESEARCH0005.

PMID: 11182888 PMC: 25775. DOI: 10.1186/gb-2001-2-2-research0005.

Faithful expression of a heterologous gene carried on an artificial macronuclear chromosome in Euplotes crassus.

Bender J, Kampfer M, Klein A Nucleic Acids Res. 1999; 27(15):3168-72.

PMID: 10454614 PMC: 148544. DOI: 10.1093/nar/27.15.3168.

Sequential excision of internal eliminated DNA sequences in the differentiating macronucleus of the hypotrichous ciliate Stylonychia lemnae.

Wen J, Maercker C, Lipps H Nucleic Acids Res. 1996; 24(22):4415-9.

PMID: 8948632 PMC: 146267. DOI: 10.1093/nar/24.22.4415.

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