Synaptonemal Complex Analysis in Spermatocytes and Oocytes of Rainbow Trout, Oncorhynchus Mykiss (Pisces, Salmonidae): the Process of Autosome and Sex Chromosome Synapsis

Overview

Journal Chromosome Res

Date 1995 May 1

PMID 7780662

Citations 9

Authors

C Oliveira

F Foresti

M G Rigolino

Y A Tabata

Affiliations

Soon will be listed here.

Abstract

The surface-spreading synaptonemal complex (SC) technique was employed to analyze spermatocytes and oocytes of rainbow trout in order to visualize the process of autosome and sex chromosome synapsis in this species. The structure of lateral elements (LEs) of the SC and the chromosome synapsis process at the stages of leptotene, zygotene and pachytene are described. Comparative analysis of SCs of spermatocytes and oocytes showed a difference in the synaptic process, i.e. in spermatocytes all LEs were synapsed before the appearance of centromeric regions in the biarmed elements, while in the oocytes some fully synapsed LEs, including the centromeric region of the biarmed elements, were found together with fully or partially unsynapsed LEs. In males the sex chromosome synapsis starts only after all autosomes have synapsed. Irregular synapses involving three or four LEs were found in 3.4% of the cells analyzed in mid or late zygotene. Multivalents were found in males and females. Some aspects of initial meiotic development and their implications in rainbow trout cytogenetics, genetics and evolution are discussed.

Citing Articles

Chromosomal characteristics and distribution of rDNA sequences in the brook trout Salvelinus fontinalis (Mitchill, 1814).

Sliwinska-Jewsiewicka A, Kucinski M, Kirtiklis L, Dobosz S, Ocalewicz K, Jankun M Genetica. 2015; 143(4):425-32.

PMID: 25958180 PMC: 4486110. DOI: 10.1007/s10709-015-9841-6.

Surface-spreading technique of meiotic cells and immunodetection of synaptonemal complex proteins in teleostean fishes.

Araya-Jaime C, Serrano E, Silva D, Yamashita M, Iwai T, Oliveira C Mol Cytogenet. 2015; 8(1):4.

PMID: 25642289 PMC: 4312464. DOI: 10.1186/s13039-015-0108-9.

Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae).

Campos-Ramos R, Harvey S, Penman D Genetica. 2008; 135(3):325-32.

PMID: 18536997 DOI: 10.1007/s10709-008-9280-8.

Morphologically differentiated sex chromosomes in neotropical freshwater fish.

de Almeida Toledo L, Foresti F Genetica. 2002; 111(1-3):91-100.

PMID: 11841192 DOI: 10.1023/a:1013768104422.

Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis.

Campos-Ramos R, Harvey S, Masabanda J, Carrasco L, Griffin D, McAndrew B Genetica. 2002; 111(1-3):143-53.

PMID: 11841163 DOI: 10.1023/a:1013707818534.

References

Howell W, Black D . Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980; 36(8):1014-5. DOI: 10.1007/BF01953855. View

Ohno S, STENIUS C, FAISST E, Zenzes M . POST-ZYGOTIC CHROMOSOMAL REARRANGEMENTS IN RAINBOW TROUT (SALMO IRIDEUS GIBBONS). Cytogenetics. 1965; 4:117-29. DOI: 10.1159/000129849. View

Goldstein P, Slaton D . The synaptonemal complexes of caenorhabditis elegans: comparison of wild-type and mutant strains and pachytene karyotype analysis of wild-type. Chromosoma. 1982; 84(4):585-97. DOI: 10.1007/BF00292857. View

Jimenez R, Burgos M, Sanchez A, de la Guardia R . Synaptonemal complex analysis of spermatocytes of Talpa occidentalis (Insectivora, Mammalia): autosomal synapsis and substaging of zygonema and pachynema. Cytogenet Cell Genet. 1990; 53(2-3):97-102. DOI: 10.1159/000132904. View

Solari A . The relationship between chromosomes and axes in the chiasmatic XY pair of the Armenian hamster (Cricetulus migratorius). Chromosoma. 1974; 48(1):89-106. DOI: 10.1007/BF00284869. View

Loidl J, Nairz K, Klein F . Meiotic chromosome synapsis in a haploid yeast. Chromosoma. 1991; 100(4):221-8. DOI: 10.1007/BF00344155. View

Loidl J . The initiation of meiotic chromosome pairing: the cytological view. Genome. 1990; 33(6):759-78. DOI: 10.1139/g90-115. View

Thorgaard G . Heteromorphic sex chromosomes in male rainbow trout. Science. 1977; 196(4292):900-2. DOI: 10.1126/science.860122. View

Carnero A, Jimenez R, Burgos M, Sanchez A, de la Guardia R . The synaptic sequence in hydroxyurea-treated spermatocytes of Pitymys duodecimcostatus (Rodentia, Microtidae). Cytogenet Cell Genet. 1991; 56(2):69-73. DOI: 10.1159/000133052. View

10.

Hartley S, Horne M . Chromosome polymorphism in the rainbow trout (Salmo gairdneri Richardson). Chromosoma. 1982; 87(5):461-8. DOI: 10.1007/BF00333467. View

11.

Grao P, Coll M, Ponsa M, Egozcue J . Trivalent behavior during prophase I in male mice heterozygous for three Robertsonian translocations: an electron-microscopic study. Cytogenet Cell Genet. 1989; 52(3-4):105-10. DOI: 10.1159/000132857. View

12.

Goldstein P, Moens P . Karyotype analysis of Ascaris lumbricoides var. suum. Male and female pachytene nuclei by 3-D reconstruction from electron microscopy of serial sections. Chromosoma. 1976; 58(2):101-11. DOI: 10.1007/BF00701353. View

13.

Loidl J, Schweizer D . Synaptonemal complexes of Xenopus laevis. J Hered. 1992; 83(4):307-9. DOI: 10.1093/oxfordjournals.jhered.a111218. View

14.

Sherman J, Herickhoff L, Stack S . Silver staining two types of meiotic nodules. Genome. 1992; 35(6):907-15. DOI: 10.1139/g92-140. View

15.

MITTWOCH U, Mahadevaiah S, Setterfield L . Pachytene pairing and oocyte numbers in mice with two single Robertsonian translocations and the male-sterile compound with monobrachial homology. Cytogenet Cell Genet. 1990; 53(2-3):144-7. DOI: 10.1159/000132916. View

16.

Greenbaum I, Hale D, Fuxa K . The mechanism of autosomal synapsis and the substaging of zygonema and pachynema from deer mouse spermatocytes. Chromosoma. 1986; 93(3):203-12. DOI: 10.1007/BF00292739. View

17.

von Wettstein D, Rasmussen S, Holm P . The synaptonemal complex in genetic segregation. Annu Rev Genet. 1984; 18:331-413. DOI: 10.1146/annurev.ge.18.120184.001555. View

18.

Wallace B, Hulten M . Meiotic chromosome pairing in the normal human female. Ann Hum Genet. 1985; 49(3):215-26. DOI: 10.1111/j.1469-1809.1985.tb01695.x. View

19.

Lin Y, Yu Q . [Electron microscopic observation of synaptonemal complexes in spermatocytes of six species of fishes]. Yi Chuan Xue Bao. 1991; 18(5):407-14. View

20.

Mahadevaiah S, Setterfield L, MITTWOCH U . Pachytene pairing and sperm counts in mice with single Robertsonian translocations and monobrachial compounds. Cytogenet Cell Genet. 1990; 53(1):26-31. DOI: 10.1159/000132889. View