Presence of CD3+CD8+Bcl-2(low) Lymphocytes Undergoing Apoptosis and Activated Macrophages in Lymph Nodes of HIV-1+ Patients

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1995 Jun 1

PMID 7778692

Citations 15

Authors

M Bofill

W Gombert

N J Borthwick

A N Akbar

J E McLaughlin

C A Lee

M A Johnson

A J Pinching

G Janossy

Affiliations

Soon will be listed here.

Abstract

Infection with human immunodeficiency virus 1 causes profound changes in the lymph nodes of infected patients. In particular, large numbers of CD8+CD45RO+ T cells infiltrate both the paracortex and the germinal centers. These cells contained the cytotoxic granule-associated protein TIA-1 but showed no detectable levels of perforin and shared the same characteristics of the expanded, activated, short-lived CD8+ population found during acute viral infections. These cells expressed low levels of Bcl-2 and are likely to be short-lived in vivo as evidenced by the direct observation of CD8+ apoptotic cells in the paracortical areas of the infected nodes. Changes in the paracortical nonlymphoid populations were also seen. There were reactive changes in the blood vessels, and the macrophage population was expanded and activated. Furthermore, apoptotic bodies were seen in the cytoplasm of the activated CD68+RFD-7+RFD-1+ macrophages pointing to the phagocytic capacity of these cells and their role in the clearance of the apoptotic cells from the tissues. These observations suggest that the persistance of CD8+ population in human immunodeficiency virus 1 infection is not a result of the presence of an abnormal CD8+ population but rather a result of an inappropriate over-stimulation of the CD8+ cells.

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References

Janossy G, Tidman N, Selby W, Thomas J, Granger S, Kung P . Human T lymphocytes of inducer and suppressor type occupy different microenvironments. Nature. 1980; 288(5786):81-4. DOI: 10.1038/288081a0. View

Hoffman R, Kung P, Hansen W, Goldstein G . Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad Sci U S A. 1980; 77(8):4914-7. PMC: 349959. DOI: 10.1073/pnas.77.8.4914. View

Modlin R, Meyer P, Hofman F, Mehlmauer M, Levy N, LUKES R . T-lymphocyte subsets in lymph nodes from homosexual men. JAMA. 1983; 250(10):1302-5. View

IOACHIM H, Lerner C, Tapper M . Lymphadenopathies in homosexual men. Relationships with the acquired immune deficiency syndrome. JAMA. 1983; 250(10):1306-9. DOI: 10.1001/jama.250.10.1306. View

Nathan C, Murray H, Wiebe M, Rubin B . Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983; 158(3):670-89. PMC: 2187114. DOI: 10.1084/jem.158.3.670. View

Fahey J, Prince H, Weaver M, Groopman J, Visscher B, Schwartz K . Quantitative changes in T helper or T suppressor/cytotoxic lymphocyte subsets that distinguish acquired immune deficiency syndrome from other immune subset disorders. Am J Med. 1984; 76(1):95-100. DOI: 10.1016/0002-9343(84)90756-3. View

Huber C, Batchelor J, Fuchs D, Hausen A, Lang A, Niederwieser D . Immune response-associated production of neopterin. Release from macrophages primarily under control of interferon-gamma. J Exp Med. 1984; 160(1):310-6. PMC: 2187425. DOI: 10.1084/jem.160.1.310. View

Gerdes J, Lemke H, Baisch H, Wacker H, Schwab U, STEIN H . Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984; 133(4):1710-5. View

Janossy G, Pinching A, Bofill M, Weber J, McLaughlin J, Ornstein M . An immunohistological approach to persistent lymphadenopathy and its relevance to AIDS. Clin Exp Immunol. 1985; 59(2):257-66. PMC: 1577142. View

10.

Biberfeld P, Bottiger B, Biberfeld G . Immunohistopathology of lymph nodes in HTLV-III infected homosexuals with persistent adenopathy or AIDS. Cancer Res. 1985; 45(9 Suppl):4665s-4670s. View

11.

Wood G, Burns B, DORFMAN R, Warnke R . In situ quantitation of lymph node helper, suppressor, and cytotoxic T cell subsets in AIDS. Blood. 1986; 67(3):596-603. View

12.

Piris M, Rivas C, Morente M, Rubio C, Martin C, Olivia H . Persistent and generalized lymphadenopathy: a lesion of follicular dendritic cells? An immunohistologic and ultrastructural study. Am J Clin Pathol. 1987; 87(6):716-24. DOI: 10.1093/ajcp/87.6.716. View

13.

Tenner-Racz K, Racz P, Schmidt H, Dietrich M, Kern P, Louie A . Immunohistochemical, electron microscopic and in situ hybridization evidence for the involvement of lymphatics in the spread of HIV-1. AIDS. 1988; 2(4):299-309. DOI: 10.1097/00002030-198808000-00010. View

14.

Hoffenbach A, Langlade-Demoyen P, Dadaglio G, Vilmer E, Michel F, Mayaud C . Unusually high frequencies of HIV-specific cytotoxic T lymphocytes in humans. J Immunol. 1989; 142(2):452-62. View

15.

Phillips A, Lee C, Elford J, Janossy G, Bofill M, Timms A . Prediction of progression to AIDS by analysis of CD4 lymphocyte counts in a haemophilic cohort. AIDS. 1989; 3(11):737-41. DOI: 10.1097/00002030-198911000-00008. View

16.

Fuchs D, Shearer G, Boswell R, Clerici M, Reibnegger G, Werner E . Increased serum neopterin in patients with HIV-1 infection is correlated with reduced in vitro interleukin-2 production. Clin Exp Immunol. 1990; 80(1):44-8. PMC: 1535232. DOI: 10.1111/j.1365-2249.1990.tb06439.x. View

17.

Muller H, Falk S, Schmidts H, STUTTE H . In situ immunophenotyping of lymphocytes/macrophages: grading of lymphadenopathy, staging and pathophysiology of HIV infection. Res Virol. 1990; 141(2):171-84. DOI: 10.1016/0923-2516(90)90019-f. View

18.

Beverley P . Is T-cell memory maintained by crossreactive stimulation?. Immunol Today. 1990; 11(6):203-5. DOI: 10.1016/0167-5699(90)90083-l. View

19.

Emilie D, Peuchmaur M, Maillot M, Crevon M, Brousse N, Delfraissy J . Production of interleukins in human immunodeficiency virus-1-replicating lymph nodes. J Clin Invest. 1990; 86(1):148-59. PMC: 296702. DOI: 10.1172/JCI114678. View

20.

Spiteri M, Poulter L . Characterization of immune inducer and suppressor macrophages from the normal human lung. Clin Exp Immunol. 1991; 83(1):157-62. PMC: 1535468. DOI: 10.1111/j.1365-2249.1991.tb05607.x. View