Two Pathogen-responsive Genes in Parsley Encode a Tyrosine-rich Hydroxyproline-rich Glycoprotein (hrgp) and an Anionic Peroxidase

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1995 May 20

PMID 7770052

Citations 12

Authors

P Kawalleck

E Schmelzer

K Hahlbrock

I E Somssich

Affiliations

Soon will be listed here.

Abstract

Two recently isolated cDNAs representing genes that are transcriptionally activated in fungus-infected parsley leaves or elicitor-treated, cultured parsley cells are shown to encode a hydroxyproline-rich glycoprotein (HRGP) and an anionic peroxidase. The deduced HRGP protein is rich in tyrosine residues, a feature also found in other pathogen- and wound-induced plant HRGPs. Expression of the peroxidase gene(s) is induced rapidly upon elicitation and precedes that of the HRGP gene. In situ hybridization experiments demonstrate the presence of HRGP and peroxidase mRNAs in parsley tissue around fungal infection sites. Peroxidase mRNA accumulation is particularly sharply restricted to plant cells directly adjacent to fungal hyphae. These results provide further evidence for an important role of specific cell wall modifications in plant defense.

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References

Johansson A, Rasmussen S, Harthill J, Welinder K . cDNA, amino acid and carbohydrate sequence of barley seed-specific peroxidase BP 1. Plant Mol Biol. 1992; 18(6):1151-61. DOI: 10.1007/BF00047718. View

Kawalleck P, Plesch G, Hahlbrock K, Somssich I . Induction by fungal elicitor of S-adenosyl-L-methionine synthetase and S-adenosyl-L-homocysteine hydrolase mRNAs in cultured cells and leaves of Petroselinum crispum. Proc Natl Acad Sci U S A. 1992; 89(10):4713-7. PMC: 49153. DOI: 10.1073/pnas.89.10.4713. View

Lagrimini L, Rothstein S . Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection. Plant Physiol. 1987; 84(2):438-42. PMC: 1056598. DOI: 10.1104/pp.84.2.438. View

Mohan R, Kolattukudy P . Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum. Plant Physiol. 1990; 92(1):276-80. PMC: 1062283. DOI: 10.1104/pp.92.1.276. View

Chen E, Seeburg P . Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985; 4(2):165-70. DOI: 10.1089/dna.1985.4.165. View

Kawalleck P, Keller H, Hahlbrock K, Scheel D, Somssich I . A pathogen-responsive gene of parsley encodes tyrosine decarboxylase. J Biol Chem. 1993; 268(3):2189-94. View

Chen J, Varner J . Isolation and characterization of cDNA clones for carrot extensin and a proline-rich 33-kDa protein. Proc Natl Acad Sci U S A. 1985; 82(13):4399-403. PMC: 390421. DOI: 10.1073/pnas.82.13.4399. View

Roberts E, Kolattukudy P . Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet. 1989; 217(2-3):223-32. DOI: 10.1007/BF02464885. View

Ayers A, Ebel J, Valent B, Albersheim P . Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976; 57(5):760-5. PMC: 542114. DOI: 10.1104/pp.57.5.760. View

10.

Lagrimini L, Burkhart W, Moyer M, Rothstein S . Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression. Proc Natl Acad Sci U S A. 1987; 84(21):7542-6. PMC: 299335. DOI: 10.1073/pnas.84.21.7542. View

11.

Showalter A, Bell J, Cramer C, Bailey J, Varner J, Lamb C . Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc Natl Acad Sci U S A. 1985; 82(19):6551-5. PMC: 391247. DOI: 10.1073/pnas.82.19.6551. View

12.

Tyson H . Relationships among amino acid sequences of animal, microbial and plant peroxidases. Theor Appl Genet. 2013; 84(5-6):643-55. DOI: 10.1007/BF00224164. View

13.

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

14.

Showalter A . Structure and function of plant cell wall proteins. Plant Cell. 1993; 5(1):9-23. PMC: 160246. DOI: 10.1105/tpc.5.1.9. View

15.

Bradley D, Kjellbom P, Lamb C . Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992; 70(1):21-30. DOI: 10.1016/0092-8674(92)90530-p. View

16.

Roberts E, Kutchan T, Kolattukudy P . Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits. Plant Mol Biol. 2013; 11(1):15-26. DOI: 10.1007/BF00016010. View

17.

Lagrimini L . Wound-induced deposition of polyphenols in transgenic plants overexpressing peroxidase. Plant Physiol. 1991; 96(2):577-83. PMC: 1080809. DOI: 10.1104/pp.96.2.577. View

18.

Corbin D, Sauer N, Lamb C . Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol. 1987; 7(12):4337-44. PMC: 368117. DOI: 10.1128/mcb.7.12.4337-4344.1987. View

19.

Showalter A, Zhou J, Rumeau D, Worst S, Varner J . Tomato extensin and extensin-like cDNAs: structure and expression in response to wounding. Plant Mol Biol. 1991; 16(4):547-65. DOI: 10.1007/BF00023421. View

20.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View