A Molecular Determinant of Human Immunodeficiency Virus Particle Assembly Located in Matrix Antigen P17

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1995 Jul 1

PMID 7769715

Citations 14

Authors

Y Morikawa

T Kishi

W H Zhang

M V Nermut

D J Hockley

I M Jones

Affiliations

Soon will be listed here.

Abstract

We report single-point mutations that are located in the matrix protein domain of the gag gene of human immunodeficiency virus type 1 and that prevent Gag particle formation. We show that mutations of p17 that abolish human immunodeficiency virus particle assembly also prevent the dimerization of p17 protein, as measured directly by a protein-protein binding assay. In the three-dimensional structure of p17, mutations that abolish dimerization are located in a single alpha helix that forms part of a fingerlike projection from one side of the molecule. Peptides derived from this region of p17 also reduce the level of p17 dimer when they are added to p17-expressing cells and compete for p17 self-association when present in protein-protein binding assays. We propose that the dimerization of the Gag precursor that occurs by the interdigitation of alpha helices on adjacent matrix molecules is a key stage in virion assembly and that the prevention of such an interaction is the molecular basis of particle misassembly.

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