Comparative Amino Acid Sequence Analysis of the Major Outer Capsid Protein (VP7) of Porcine Rotaviruses with G3 and G5 Serotype Specificities Isolated in Venezuela and Argentina

Overview

Journal Arch Virol

Specialty Microbiology

Date 1995 Jan 1

PMID 7733818

Citations 17

Authors

M Ciarlet

J E Ludert

F Liprandi

Affiliations

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Abstract

Seven porcine group A rotavirus strains isolated in Venezuela were shown to be antigenically related to serotype G3 (five strains) or to serotype G5 (two strains), whereas two strains isolated in Argentina were classified as serotype G5. The serological classification of eight of these strains was confirmed by sequence analysis of the gene encoding the VP7 glycoprotein. A high degree of homology was observed among strains belonging to the same G serotype, although some variations in the serotype-specific regions were detected among different strains. Comparison with the published VP7 amino acid sequences of serotype G3 indicated that most porcine rotavirus strains are more closely related to each other and to human rotavirus strains than to rotavirus strains isolated from other species. Amino acid sequence comparison among serotype G5 porcine strains revealed that Venezuelan porcine isolates were more closely related to the American strain OSU, while the Argentinian strains had a higher similarity to the Australian strain TRF-41. This report confirms the worldwide distribution of these G serotypes among the porcine population.

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References

Bellinzoni R, Mattion N, Burrone O, Gonzalez A, La Torre J, Scodeller E . Isolation of group A swine rotaviruses displaying atypical electropherotypes. J Clin Microbiol. 1987; 25(5):952-4. PMC: 266131. DOI: 10.1128/jcm.25.5.952-954.1987. View

Taniguchi K, Urasawa T, URASAWA S . Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology. 1994; 200(2):390-400. DOI: 10.1006/viro.1994.1203. View

Browning G, Chalmers R, Fitzgerald T, Snodgrass D . Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol. 1991; 72 ( Pt 5):1059-64. DOI: 10.1099/0022-1317-72-5-1059. View

Offit P, Blavat G . Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986; 57(1):376-8. PMC: 252740. DOI: 10.1128/JVI.57.1.376-378.1986. View

Gorziglia M, Nishikawa K, Hoshino Y, Taniguchi K . Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. J Virol. 1990; 64(1):414-8. PMC: 249118. DOI: 10.1128/JVI.64.1.414-418.1990. View

Green K, Midthun K, Gorziglia M, Hoshino Y, Kapikian A, Chanock R . Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987; 161(1):153-9. DOI: 10.1016/0042-6822(87)90181-4. View

Huang J, Nagesha H, HOLMES I . Comparative sequence analysis of VP4s from five Australian porcine rotaviruses: implication of an apparent new P type. Virology. 1993; 196(1):319-27. DOI: 10.1006/viro.1993.1482. View

Ruiz A, Lopez I, Lopez S, Espejo R, Arias C . Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. J Virol. 1988; 62(11):4331-6. PMC: 253868. DOI: 10.1128/JVI.62.11.4331-4336.1988. View

Caust J, Dyall-Smith M, Lazdins I, HOLMES I . Glycosylation, an important modifier of rotavirus antigenicity. Arch Virol. 1987; 96(3-4):123-34. DOI: 10.1007/BF01320955. View

10.

Browning G, Fitzgerald T, Chalmers R, Snodgrass D . A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol. 1991; 29(9):2043-6. PMC: 270257. DOI: 10.1128/jcm.29.9.2043-2046.1991. View

11.

Kang S, Saif L, Miller K . Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol. 1989; 27(12):2744-50. PMC: 267120. DOI: 10.1128/jcm.27.12.2744-2750.1989. View

12.

Higgins D, Sharp P . Fast and sensitive multiple sequence alignments on a microcomputer. Comput Appl Biosci. 1989; 5(2):151-3. DOI: 10.1093/bioinformatics/5.2.151. View

13.

Rosen B, Parwani A, Lopez S, Flores J, Saif L . Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol. 1994; 32(2):311-7. PMC: 263030. DOI: 10.1128/jcm.32.2.311-317.1994. View

14.

Estes M, Cohen J . Rotavirus gene structure and function. Microbiol Rev. 1989; 53(4):410-49. PMC: 372748. DOI: 10.1128/mr.53.4.410-449.1989. View

15.

Glass R, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian A . Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985; 141(2):292-8. DOI: 10.1016/0042-6822(85)90260-0. View

16.

Li B, Gorziglia M . VP4 serotype of the Gottfried strain of porcine rotavirus. J Clin Microbiol. 1993; 31(11):3075-7. PMC: 266227. DOI: 10.1128/jcm.31.11.3075-3077.1993. View

17.

Liprandi F, Lopez G, Rodriguez I, Hidalgo M, Ludert J, Mattion N . Monoclonal antibodies to the VP6 of porcine subgroup I rotaviruses reactive with subgroup I and non-subgroup I non-subgroup II strains. J Gen Virol. 1990; 71 ( Pt 6):1395-8. DOI: 10.1099/0022-1317-71-6-1395. View

18.

Mattion N, Bellinzoni R, Blackhall J, La Torre J, Scodeller E . Antigenic characterization of swine rotaviruses in Argentina. J Clin Microbiol. 1989; 27(4):795-8. PMC: 267425. DOI: 10.1128/jcm.27.4.795-798.1989. View

19.

Gouvea V, de Castro L, Timenetsky M, Greenberg H, Santos N . Rotavirus serotype G5 associated with diarrhea in Brazilian children. J Clin Microbiol. 1994; 32(5):1408-9. PMC: 263717. DOI: 10.1128/jcm.32.5.1408-1409.1994. View

20.

Huang J, Nagesha H, Dyall-Smith M, HOLMES I . Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Arch Virol. 1989; 109(3-4):173-83. DOI: 10.1007/BF01311079. View