Fatty Acid Transport by the Lipophilic Bacterium Nocardia Asteroides

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 May 1

PMID 770452

Citations 2

Authors

R Calmes

S J DEAL

Affiliations

Soon will be listed here.

Abstract

Hexadecanoate was translocated in Nocardia asteroides by a constitutive transport system(s), which transported short, medium, and long-chain fatty acids. Inhibition of hexadenocanoate transport by homologues suggested that at least two systems are present: one specific for short-chain fatty acids and the other specific for medium- and long-chain fatty acids. Saturation kinetics typical of a carrier-mediated transport system (Kt = 870 muM)were observed, and concentration of fatty acids against a gradient was achieved. Inhibitor studies indicated that free sulfhydryl groups, a functional respiratory chain, and energy are required for translocation. Efflux of [14C]hexadecanoate in the presence of excess unlabeled hexadecanoate or 2,4-dinitrophenol and the cytoplasmic localization of acyl-coenzyme A synthetase (acid:coenzyme A ligase [adenosine monophosphate]; EC 6.2.1.3) (Calmes and Deal, 1973) are consistent with the hypothesis that fatty acids are transported and released intracellularly as free fatty acids.

Citing Articles

Involvement of phosphoenolpyruvate in the catabolism of caries-conducive disaccharides by Streptococcus mutans: lactose transport.

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PMID: 246429 PMC: 422282. DOI: 10.1128/iai.19.3.934-942.1978.

Energy-dependent incorporation of sphingolipid precursors and fatty acids in Bacteriodes melaninogenicus.

Lev M, Milford A J Bacteriol. 1977; 130(1):445-54.

PMID: 15984 PMC: 235223. DOI: 10.1128/jb.130.1.445-454.1977.

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