Fatty Acid Activation by a Lipophilic Bacterium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Apr 1

PMID 4572713

Citations 1

Authors

R Calmes

S J DEAL

Affiliations

Soon will be listed here.

Abstract

Cell-free extracts of Nocardia asteroides activated saturated fatty acids from octanoate to octadecanoate, plus docosanoate; maximal activation occurred with dodecanoate. No activation of short-chain fatty acids was observed. The activating enzyme, characterized as an acyl-coenzyme A (Co A) synthetase (acid: Co A ligase [adenosine monophosphate]; EC 6.2.1.3), was localized in the cytoplasm of the cells and had absolute requirements for Co A, adenosine 5'-triphosphate, and Mg(2+). Kinetic data suggested that N. asteroides possessed at least two synthetases: one specific for short-chain fatty acids, and the other specific for medium- and long-chain fatty acids.

Citing Articles

Fatty acid transport by the lipophilic bacterium Nocardia asteroides.

Calmes R, DEAL S J Bacteriol. 1976; 126(2):751-7.

PMID: 770452 PMC: 233210. DOI: 10.1128/jb.126.2.751-757.1976.

References

Cronan J, VAGELOS P . Metabolism and function of the membrane phospholipids of Escherichia coli. Biochim Biophys Acta. 1972; 265(1):25-60. DOI: 10.1016/0304-4157(72)90018-4. View

Silliker J, RITTENBERG S . Studies on the aerobic oxidation of fatty acids by bacteria, I. The nature of the enzymes, constitutive or adaptive. J Bacteriol. 1951; 61(6):653-9. PMC: 386061. DOI: 10.1128/jb.61.6.653-659.1951. View

Kornberg A, PRICER Jr W . Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids. J Biol Chem. 1953; 204(1):329-43. View

Senior J, Isselbacher K . Activation of long-chain fatty acids by rat-gut mucosa. Biochim Biophys Acta. 1960; 44:399-400. DOI: 10.1016/0006-3002(60)91594-8. View

Eaton N . New press for disruption of microorganisms. J Bacteriol. 1962; 83:1359-60. PMC: 279462. DOI: 10.1128/jb.83.6.1359-1360.1962. View

Lennarz W . A long-chain fatty acid acyl-coA synthetase in Bacillus megaterium. Biochim Biophys Acta. 1963; 73:335-7. DOI: 10.1016/0006-3002(63)90320-2. View

Davis J . MICROBIAL INCORPORATION OF FATTY ACIDS DERIVED FROM N-ALKANES INTO GLYCERIDES AND WAXES. Appl Microbiol. 1964; 12:210-4. PMC: 1058101. DOI: 10.1128/am.12.3.210-214.1964. View

Randerath K, RANDERATH E . ION-EXCHANGE CHROMATOGRAPHY OF NUCLEOTIDES ON POLY-(ETHYLENEIMINE)-CELLULOSE THIN LAYERS. J Chromatogr. 1964; 16:111-25. DOI: 10.1016/s0021-9673(01)82445-6. View

Massaro E, Lennarz W . THE PARTIAL PURIFICATION AND CHARACTERIZATION OF A BACTERIAL FATTY ACYL COENZYME A SYNTHETASE. Biochemistry. 1965; 4:85-90. DOI: 10.1021/bi00877a015. View

10.

Ailhaud G, Sarda L, DESNUELLE P . [Formation of hydroxamates of long chain fatty acids by a subcellular fraction of intestinal mucosa]. Biochim Biophys Acta. 1962; 59:261-72. DOI: 10.1016/0006-3002(62)90174-9. View

11.

Salanitro J, Wegener W . Growth of Escherichia coli on short-chain fatty acids: growth characteristics of mutants. J Bacteriol. 1971; 108(2):885-92. PMC: 247156. DOI: 10.1128/jb.108.2.885-892.1971. View

12.

Overath P, Raufuss E . The induction of the enzymes of fatty acid degradation in Escherichia coli. Biochem Biophys Res Commun. 1967; 29(1):28-33. DOI: 10.1016/0006-291x(67)90535-9. View

13.

PANDE S, Mead J . Long chain fatty acid activation in subcellular preparations from rat liver. J Biol Chem. 1968; 243(2):352-61. View

14.

Weeks G, Shapiro M, BURNS R, WAKIL S . Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli. J Bacteriol. 1969; 97(2):827-36. PMC: 249767. DOI: 10.1128/jb.97.2.827-836.1969. View

15.

Overath P, Pauli G, Schairer H . Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old-mutations, and the isolation of regulatory mutants. Eur J Biochem. 1969; 7(4):559-74. View

16.

Henneberry R, Cox C . Beta-oxidation of fatty acids by Leptospira. Can J Microbiol. 1970; 16(1):41-5. DOI: 10.1139/m70-007. View

17.

Samuel D, Estroumza J, Ailhaud G . Partial purification and properties of acyl-CoA synthetase of Escherichia coli. Eur J Biochem. 1970; 12(3):576-82. DOI: 10.1111/j.1432-1033.1970.tb00889.x. View

18.

Kaback H . Transport. Annu Rev Biochem. 1970; 39:561-98. DOI: 10.1146/annurev.bi.39.070170.003021. View

19.

Trust T, Millis N . Activation of the C2to C19 monocarboxylic acids by Pseudomonas. J Bacteriol. 1971; 105(3):1216-8. PMC: 248566. DOI: 10.1128/jb.105.3.1216-1218.1971. View

20.

Klein K, Steinberg R, Fiethen B, Overath P . Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur J Biochem. 1971; 19(3):442-50. DOI: 10.1111/j.1432-1033.1971.tb01334.x. View