Immunohistochemical Study of Cell Proliferation and Differentiation in Epidermis of Mice After Administration of Cholera Toxin

Overview

Journal Arch Dermatol Res

Specialty Dermatology

Date 1993 Jan 1

PMID 7682399

Citations 2

Authors

S A Rahman

S Tsuyama

Affiliations

Soon will be listed here.

Abstract

Cholera toxin causes reversible epidermal hyperplasia. We observed maximal thickness of the epidermis on the fourth day after treatment and a return to pretreatment values by day 7. The increase in thickness occurred in the basal and intermediate layers, with these layers becoming two to three times thicker than those of normal epidermis. The time sequence of epidermal proliferation was studied using bromodeoxyuridine (BrdU) labelling. We observed a maximum number of labelled basal cells within the first 24 h. Only a few cells were labelled 7 days after toxin injection. Griffonia simplicifolia-IB4 (GSA-IB4), Ulex europaeus-I (UEA-I) and Griffonia simplicifolia-II (GSA-II) lectins were used for the analysis of epidermal cell differentiation in the tissue sections. To study keratinocyte differentiation, further immunological staining was performed using two anticytokeratin antibodies, PKK2 and PKK3 mouse monoclonal antibodies. From the immunocytochemical results, we conclude that synchronous differentiation of the epidermis occurs after cholera toxin administration.

Citing Articles

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization.

Dolbeare F Histochem J. 1996; 28(8):531-75.

PMID: 8894660 DOI: 10.1007/BF02331377.

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part II: Oncology, chemotherapy and carcinogenesis.

Dolbeare F Histochem J. 1995; 27(12):923-64.

PMID: 8789396

References

. Ultrastructural localization of lectin-binding sites in normal skin. J Invest Dermatol. 1990; 94(4):465-70. DOI: 10.1111/1523-1747.ep12874590. View

Kuroki T, Chida K, Munakata K, Murakami Y . Cholera toxin, a potent inducer of epidermal hyperplasia but with no tumor promoting activity in mouse skin carcinogenesis. Biochem Biophys Res Commun. 1986; 137(1):486-92. DOI: 10.1016/0006-291x(86)91236-2. View

Ku W, BERNSTEIN I . Preliminary characterization of cell surface glycoproteins associated with epidermal differentiation in the newborn rat. Biochem Biophys Res Commun. 1985; 132(1):269-76. DOI: 10.1016/0006-291x(85)91018-6. View

Nowakowski R, Lewin S, Miller M . Bromodeoxyuridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined population. J Neurocytol. 1989; 18(3):311-8. DOI: 10.1007/BF01190834. View

Moll R, Franke W, Volc-Platzer B, Krepler R . Different keratin polypeptides in epidermis and other epithelia of human skin: a specific cytokeratin of molecular weight 46,000 in epithelia of the pilosebaceous tract and basal cell epitheliomas. J Cell Biol. 1982; 95(1):285-95. PMC: 2112346. DOI: 10.1083/jcb.95.1.285. View

Reano A, Faure M, Jacques Y, Reichert U, Schaefer H, Thivolet J . Lectins as markers of human epidermal cell differentiation. Differentiation. 1982; 22(3):205-10. DOI: 10.1111/j.1432-0436.1982.tb01252.x. View

Trent J, Gerner E, Broderick R, Crossen P . Cell cycle analysis using bromodeoxyuridine: comparison of methods for analysis of total cell transit time. Cancer Genet Cytogenet. 1986; 19(1-2):43-50. DOI: 10.1016/0165-4608(86)90370-5. View

Bell C, Skerrow C . Factors affecting the binding of lectins to normal human skin. Br J Dermatol. 1984; 111(5):517-26. DOI: 10.1111/j.1365-2133.1984.tb06620.x. View

Brabec R, Peters B, BERNSTEIN I, Gray R, Goldstein I . Differential lectin binding to cellular membranes in the epidermis of the newborn rat. Proc Natl Acad Sci U S A. 1980; 77(1):477-9. PMC: 348294. DOI: 10.1073/pnas.77.1.477. View

10.

Gratzner H . Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218(4571):474-5. DOI: 10.1126/science.7123245. View

11.

Takeda M, Obara N, Suzuki Y . Keratin filaments of epithelial and taste-bud cells in the circumvallate papillae of adult and developing mice. Cell Tissue Res. 1990; 260(1):41-8. DOI: 10.1007/BF00297488. View

12.

Schweizer J, Winter H . Keratin polypeptide analysis in fetal and in terminally differentiating newborn mouse epidermis. Differentiation. 1982; 22(1):19-24. DOI: 10.1111/j.1432-0436.1982.tb01219.x. View

13.

Miller M, Nowakowski R . Use of bromodeoxyuridine-immunohistochemistry to examine the proliferation, migration and time of origin of cells in the central nervous system. Brain Res. 1988; 457(1):44-52. DOI: 10.1016/0006-8993(88)90055-8. View

14.

Wolff H, Gnas W . Immunocytochemical detection of in vitro incorporated 5-bromodeoxyuridine in paraffin sections of human skin. Arch Dermatol Res. 1989; 281(3):209-12. DOI: 10.1007/BF00456396. View

15.

Zieske J, BERNSTEIN I . Modification of cell surface glycoprotein: addition of fucosyl residues during epidermal differentiation. J Cell Biol. 1982; 95(2 Pt 1):626-31. PMC: 2112967. DOI: 10.1083/jcb.95.2.626. View

16.

Kuroki T . Induction by cholera toxin of synchronous divisions in vivo in the epidermis resulting in hyperplasia. Proc Natl Acad Sci U S A. 1981; 78(11):6958-62. PMC: 349172. DOI: 10.1073/pnas.78.11.6958. View