Differential Lectin Binding to Cellular Membranes in the Epidermis of the Newborn Rat

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 Jan 1

PMID 6928640

Citations 37

Authors

R K Brabec

B P Peters

I A BERNSTEIN

R H Gray

I J Goldstein

Affiliations

Soon will be listed here.

Abstract

Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex europaeus exhibiting specificity for alpha-L-fucosyl units outlines the surface of spinous cells, and a second lectin from B. simplicifolia, with specificity for N-acetyl-D-glucosamine, labels the cornified cells. Appropriate blocking experiments have confirmed the specific nature of the binding.

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