Alpha 2.0
Login Register
» Articles » PMID: 7615720

Evaluation of Staph ID 32 System and Staph-Zym System for Identification of Coagulase-negative Staphylococci

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1995 May 1
PMID 7615720
Citations 23
Authors
J Renneberg
K Rieneck
E Gutschik
Affiliations
Soon will be listed here.
Abstract

The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated. A total of 200 staphylococcal isolates, including strains from both the American Type Culture Collection and the Czechoslovak Collection of Microorganisms as well as 89 clinical isolates, were used in tests of all three identification systems. The Staph ID 32 API system identified from 50 to 100% of the reference strains and 82.1% of the clinical isolates correctly. The Staph-Zym system identified from 90 to 100% of the reference strains and 82.1% of the clinical isolates correctly. Most misidentifications were of minor importance, but in both systems major failures appeared (Staphylococcus aureus was identified as a coagulase-negative staphylococcus). Both systems needed backup from a reference laboratory to determine if two isolates were of the same strain.

Citing Articles

Using Protein Fingerprinting for Identifying and Discriminating Methicillin Resistant Isolates from Inpatient and Outpatient Clinics.

Elbehiry A, Marzouk E, Moussa I, Anagreyyah S, Alghamdi A, Alqarni A Diagnostics (Basel). 2023; 13(17).

PMID: 37685363 PMC: 10486511. DOI: 10.3390/diagnostics13172825.

Comparison of the Efficacy of Intramammary or Injectable Antibiotic Administration against Staphylococcal Mastitis in Ewes.

Vasileiou N, Fthenakis G, Mavrogianni V Pathogens. 2022; 11(10).

PMID: 36297221 PMC: 9607280. DOI: 10.3390/pathogens11101164.

Clinical characteristics of Staphylococcus epidermidis: a systematic review.

Ebrahimzadeh Namvar A, Bastarahang S, Abbasi N, Ghehi G, Farhadbakhtiarian S, Arezi P GMS Hyg Infect Control. 2014; 9(3):Doc23.

PMID: 25285267 PMC: 4184040. DOI: 10.3205/dgkh000243.

Coagulase-negative staphylococci.

Becker K, Heilmann C, Peters G Clin Microbiol Rev. 2014; 27(4):870-926.

PMID: 25278577 PMC: 4187637. DOI: 10.1128/CMR.00109-13.

Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system.

Kim S, Shin J, Mok J, Kim S, Song S, Kim H Biomed Res Int. 2014; 2014:250408.

PMID: 24977144 PMC: 4058107. DOI: 10.1155/2014/250408.

References
1.
Langlois B, Harmon R, Akers K . Identification of Staphylococcus species of bovine origin with the DMS Staph-Trac system. J Clin Microbiol. 1984; 20(2):227-30. PMC: 271292. DOI: 10.1128/jcm.20.2.227-230.1984. View
2.
Archer G, Karchmer A, Vishniavsky N, Johnston J . Plasmid-pattern analysis for the differentiation of infecting from noninfecting Staphylococcus epidermidis. J Infect Dis. 1984; 149(6):913-20. DOI: 10.1093/infdis/149.6.913. View
3.
Watts J, Owens W, Nickerson S . Evaluation of the Minitek gram-positive set for identification of staphylococci isolated from the bovine mammary gland. J Clin Microbiol. 1986; 23(5):873-5. PMC: 268740. DOI: 10.1128/jcm.23.5.873-875.1986. View
4.
Watts J, Owens W, Nickerson S . Identification of staphylococci from bovine udders: evaluation of the API 20GP system. Can J Microbiol. 1986; 32(4):359-61. DOI: 10.1139/m86-069. View
5.
Watts J, Nickerson S . A comparison of the STAPH-Ident and STAPH-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders. Vet Microbiol. 1986; 12(2):179-87. DOI: 10.1016/0378-1135(86)90079-9. View