Evidence of Post-transcriptional Regulation of L-selectin Gene Expression in Rat Lymphoid Cells

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1995 Jun 1

PMID 7543874

Citations 1

Authors

R Sackstein

L Meng

X M Xu

Y H Chin

Affiliations

Soon will be listed here.

Abstract

Early investigations of lymphocyte migration in the rat operationally identified a lymphocyte membrane protein, designated 'A.11', which mediates lymphocyte adherence to lymph node (LN) high endothelial venules (HEV). To determine the primary structure of A.11 and examine its expression in lymphoid cells, we constructed an expression phage cDNA library of rat thoracic duct lymphocytes (TDL) and performed screening by immunoselection (utilizing an anti-A.11 polyclonal antiserum) as well as by hybridization selection. We have isolated a approximately 1.6 kb clone, RS-2, and sequencing revealed that it encodes rat L-selectin. The clone contains the complete coding sequence, a 105-bp 5' untranslated region and a 359-bp 3' untranslated region. Transfection of RS-2 cDNA into 70Z/3 cells conferred binding to HEV concomitant with expression of A.11, providing direct evidence that A.11 is rat L-selectin. Metabolic radiolabelling studies revealed that thymocytes synthesize markedly less L-selectin than do TDL or LN lymphocytes. However, Northern blot studies using RS-2 as a probe indicate that thymocytes possess more L-selectin RNA than does TDL. Together, these data provide evidence that post-transcriptional events contribute to regulation of L-selectin expression in thymocytes.

Citing Articles

Glycosyltransferase-programmed stereosubstitution (GPS) to create HCELL: engineering a roadmap for cell migration.

Sackstein R Immunol Rev. 2009; 230(1):51-74.

PMID: 19594629 PMC: 4306344. DOI: 10.1111/j.1600-065X.2009.00792.x.

References

Chin Y, Carey G, Woodruff J . Lymphocyte recognition of lymph node high endothelium. V. Isolation of adhesion molecules from lysates of rat lymphocytes. J Immunol. 1983; 131(3):1368-74. View

Gallatin W, Weissman I, Butcher E . A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature. 1983; 304(5921):30-4. DOI: 10.1038/304030a0. View

Gubler U, HOFFMAN B . A simple and very efficient method for generating cDNA libraries. Gene. 1983; 25(2-3):263-9. DOI: 10.1016/0378-1119(83)90230-5. View

Sackstein R, Colten H . Molecular regulation of MHC class III (C4 and factor B) gene expression in mouse peritoneal macrophages. J Immunol. 1984; 133(3):1618-26. View

Rasmussen R, Chin Y, Woodruff J, Easton T . Lymphocyte recognition of lymph node high endothelium. VII. Cell surface proteins involved in adhesion defined by monoclonal anti-HEBFLN (A.11) antibody. J Immunol. 1985; 135(1):19-24. View

Chin Y, Rasmussen R, Woodruff J, Easton T . A monoclonal anti-HEBFPP antibody with specificity for lymphocyte surface molecules mediating adhesion to Peyer's patch high endothelium of the rat. J Immunol. 1986; 136(7):2556-61. View

Wilson A, Scollay R, Reichert R, Butcher E, Weissman I, Shortman K . The correlation of lectin-stimulated proliferation and cytotoxicity in murine thymocytes with expression of the MEL-14-defined homing receptor. J Immunol. 1987; 138(2):352-7. View

SIEGELMAN M, van de Rijn M, Weissman I . Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains. Science. 1989; 243(4895):1165-72. DOI: 10.1126/science.2646713. View

Lasky L, Singer M, Yednock T, Dowbenko D, Fennie C, Rodriguez H . Cloning of a lymphocyte homing receptor reveals a lectin domain. Cell. 1989; 56(6):1045-55. DOI: 10.1016/0092-8674(89)90637-5. View

10.

Camerini D, James S, Stamenkovic I, Seed B . Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989; 342(6245):78-82. DOI: 10.1038/342078a0. View

11.

Jung T, Dailey M . Rapid modulation of homing receptors (gp90MEL-14) induced by activators of protein kinase C. Receptor shedding due to accelerated proteolytic cleavage at the cell surface. J Immunol. 1990; 144(8):3130-6. View

12.

Tedder T, Matsuyama T, Rothstein D, Schlossman S, Morimoto C . Human antigen-specific memory T cells express the homing receptor (LAM-1) necessary for lymphocyte recirculation. Eur J Immunol. 1990; 20(6):1351-5. DOI: 10.1002/eji.1830200622. View

13.

van de Rijn M, Weissman I, Siegelman M . Biosynthesis pathway of gp90MEL-14, the mouse lymph node-specific homing receptor. J Immunol. 1990; 145(5):1477-82. View

14.

Chin Y, Cai J, Johnson K . Lymphocyte adhesion to cultured Peyer's patch high endothelial venule cells is mediated by organ-specific homing receptors and can be regulated by cytokines. J Immunol. 1990; 145(11):3669-77. View

15.

Terstappen L, Huang S, Picker L . Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood. 1992; 79(3):666-77. View

16.

Chin Y, Cai J, Xu X . Tissue-specific homing receptor mediates lymphocyte adhesion to cytokine-stimulated lymph node high endothelial venule cells. Immunology. 1991; 74(3):478-83. PMC: 1384643. View

17.

Watanabe T, Song Y, Hirayama Y, Tamatani T, Kuida K, Miyasaka M . Sequence and expression of a rat cDNA for LECAM-1. Biochim Biophys Acta. 1992; 1131(3):321-4. DOI: 10.1016/0167-4781(92)90033-v. View

18.

Tamatani T, Kitamura F, Kuida K, Shirao M, Mochizuki M, Suematsu M . Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera. Eur J Immunol. 1993; 23(9):2181-8. DOI: 10.1002/eji.1830230920. View

19.

Prystas E, Parker C, Holguin M, Bohnsack J . Aberrant glycosylation of L-selectin on the lymphocytes of chronic lymphocytic leukemia. Leukemia. 1993; 7(9):1355-62. View

20.

Sackstein R . Physiologic migration of lymphocytes to lymph nodes following bone marrow transplantation: role in immune recovery. Semin Oncol. 1993; 20(5 Suppl 6):34-9. View