Chemical Analysis and Biological Testing of a Polar Fraction of Ambient Air, Diesel Engine, and Gasoline Engine Particulate Extracts

Overview

Journal Environ Health Perspect

Date 1994 Oct 1

PMID 7529708

Citations 5

Authors

M Strandell

S Zakrisson

T Alsberg

R Westerholm

L Winquist

U Rannug

Affiliations

Soon will be listed here.

Abstract

Extracts of gasoline and diesel vehicle exhaust and ambient air particles were fractionated into five fractions according to polarity on a silica gel column. Two medium polar fractions showing high genotoxic activity in the Ames test were further subfractionated, using normal-phase high-performance liquid chromatography. Chemical analyses were performed by means of gas chromatography combined with mass spectrometry and flame ionization and detection. The crude extracts, fractions, and subfractions were assayed with the Ames test, with and without S9, and the most abundant compounds in the subfractions are reported.

Citing Articles

Ecotoxicity and genotoxicity assessment of exhaust particulates from diesel-powered buses.

Kovats N, Acs A, Ferincz A, Kovacs A, Horvath E, Kakasi B Environ Monit Assess. 2013; 185(10):8707-13.

PMID: 23609923 DOI: 10.1007/s10661-013-3206-3.

Sampling and analytical methods for assessing the levels of organic pollutants in the atmosphere: PAH, phthalates and psychotropic substances: a short review.

Cecinato A, Balducci C, Mastroianni D, Perilli M Environ Sci Pollut Res Int. 2012; 19(6):1915-26.

PMID: 22767289 DOI: 10.1007/s11356-012-0959-0.

The variation of street air levels of PAH and other mutagenic PAC in relation to regulations of traffic emissions and the impact of atmospheric processes.

Nielsen T, Feilberg A, Binderup M Environ Sci Pollut Res Int. 2008; 6(3):133-7.

PMID: 19009386 DOI: 10.1007/BF02987613.

Bioassay-directed fractionation and salmonella mutagenicity of automobile and forklift diesel exhaust particles.

DeMarini D, Brooks L, Warren S, Kobayashi T, Gilmour M, Singh P Environ Health Perspect. 2004; 112(8):814-9.

PMID: 15175166 PMC: 1242006. DOI: 10.1289/ehp.6578.

Future research needs associated with the assessment of potential human health risks from exposure to toxic ambient air pollutants.

Moller L, Schuetzle D, Autrup H Environ Health Perspect. 1994; 102 Suppl 4:193-210.

PMID: 7529703 PMC: 1566924. DOI: 10.1289/ehp.102-1566924.

References

Ames B, McCann J, Yamasaki E . Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975; 31(6):347-64. DOI: 10.1016/0165-1161(75)90046-1. View

Kincaid R, MILLER W, Jensen L, Hampton D, Neathery M, GENTRY R . Effect of high amounts of dietary zinc and age upon tissue zinc in young chicks. Poult Sci. 1976; 55(5):1954-7. DOI: 10.3382/ps.0551954. View

Mass H, Ali I, Velez W, Santiago O . Hemodynamics of heartworm infection. Ann N Y Acad Sci. 1992; 653:206-10. DOI: 10.1111/j.1749-6632.1992.tb19648.x. View

Manabe Y, Kinouchi T, Ohnishi Y . Identification and quantification of highly mutagenic nitroacetoxypyrenes and nitrohydroxypyrenes in diesel-exhaust particles. Mutat Res. 1985; 158(1-2):3-18. DOI: 10.1016/0165-1218(85)90092-8. View

Matsumoto H, Inoue K . Mutagenicity of a polar portion in the neutral fraction separated from organic extracts of airborne particulates. Arch Environ Contam Toxicol. 1987; 16(4):409-16. DOI: 10.1007/BF01055261. View