Expression of Rat Brain Nitric Oxide Synthase in Baculovirus-infected Insect Cells and Characterization of the Purified Enzyme

Overview

Journal Biochem J

Specialty Biochemistry

Date 1994 Dec 15

PMID 7529491

Citations 13

Authors

C Harteneck

P Klatt

K Schmidt

B Mayer

Affiliations

Soon will be listed here.

Abstract

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.

Citing Articles

Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells.

Eroglu E, Hallstrom S, Bischof H, Opelt M, Schmidt K, Mayer B Nitric Oxide. 2017; 70:59-67.

PMID: 28882669 PMC: 6002809. DOI: 10.1016/j.niox.2017.09.001.

Interaction between neuronal nitric-oxide synthase and tetrahydrobiopterin revisited: studies on the nature and mechanism of tight pterin binding.

Heine C, Kolesnik B, Schmidt R, Werner E, Mayer B, Gorren A Biochemistry. 2014; 53(8):1284-95.

PMID: 24512289 PMC: 3944803. DOI: 10.1021/bi401307r.

Inhibition of neuronal nitric oxide synthase by 6-nitrocatecholamines, putative reaction products of nitric oxide with catecholamines under oxidative stress conditions.

Palumbo A, Astarita G, dIschia M Biochem J. 2001; 356(Pt 1):105-10.

PMID: 11336641 PMC: 1221817. DOI: 10.1042/0264-6021:3560105.

Suramin and the suramin analogue NF307 discriminate among calmodulin-binding sites.

Klinger M, Bofill-Cardona E, Mayer B, Nanoff C, Freissmuth M, Hohenegger M Biochem J. 2001; 355(Pt 3):827-33.

PMID: 11311147 PMC: 1221800. DOI: 10.1042/bj3550827.

Nitric oxide-induced autoinhibition of neuronal nitric oxide synthase in the presence of the autoxidation-resistant pteridine 5-methyltetrahydrobiopterin.

Gorren A, Schrammel A, Riethmuller C, Schmidt K, Koesling D, Werner E Biochem J. 2000; 347(Pt 2):475-84.

PMID: 10749677 PMC: 1220980. DOI: 10.1042/0264-6021:3470475.

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