Brain Nitric Oxide Synthase is a Haemoprotein

Overview

Journal Biochem J

Specialty Biochemistry

Date 1992 Nov 15

PMID 1280109

Citations 23

Authors

P Klatt

K Schmidt

B Mayer

Affiliations

Soon will be listed here.

Abstract

Brain nitric oxide (NO) synthase showed pyridine haemochrome spectra typical of ferroprotoporphyrin IX-containing enzymes. The haem content of purified NO synthase was in the range 0.7-0.9 mol/mol of 160 kDa subunit. In the presence of CO, NO, KCN and miconazole, the L-citrulline-forming activity of NO synthase was markedly diminished, demonstrating that enzyme-bound haem is involved in enzymic NO synthesis.

Citing Articles

The Protective Role of Carbon Monoxide (CO) Produced by Heme Oxygenases and Derived from the CO-Releasing Molecule CORM-2 in the Pathogenesis of Stress-Induced Gastric Lesions: Evidence for Non-Involvement of Nitric Oxide (NO).

Magierowska K, Magierowski M, Surmiak M, Adamski J, Mazur-Bialy A, Pajdo R Int J Mol Sci. 2016; 17(4):442.

PMID: 27023525 PMC: 4848898. DOI: 10.3390/ijms17040442.

Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage.

Magierowska K, Magierowski M, Hubalewska-Mazgaj M, Adamski J, Surmiak M, Sliwowski Z PLoS One. 2015; 10(10):e0140493.

PMID: 26460608 PMC: 4604159. DOI: 10.1371/journal.pone.0140493.

Structural studies of constitutive nitric oxide synthases with diatomic ligands bound.

Li H, Igarashi J, Jamal J, Yang W, Poulos T J Biol Inorg Chem. 2006; 11(6):753-68.

PMID: 16804678 DOI: 10.1007/s00775-006-0123-8.

Recruitment of governing elements for electron transfer in the nitric oxide synthase family.

Jachymova M, Martasek P, Panda S, Roman L, Panda M, Shea T Proc Natl Acad Sci U S A. 2005; 102(44):15833-8.

PMID: 16249336 PMC: 1276075. DOI: 10.1073/pnas.0506522102.

Stopped-flow kinetic studies of electron transfer in the reductase domain of neuronal nitric oxide synthase: re-evaluation of the kinetic mechanism reveals new enzyme intermediates and variation with cytochrome P450 reductase.

Knight K, Scrutton N Biochem J. 2002; 367(Pt 1):19-30.

PMID: 12079493 PMC: 1222855. DOI: 10.1042/BJ20020667.

References

FULCO A . P450BM-3 and other inducible bacterial P450 cytochromes: biochemistry and regulation. Annu Rev Pharmacol Toxicol. 1991; 31:177-203. DOI: 10.1146/annurev.pa.31.040191.001141. View

Forstermann U, Schmidt H, Pollock J, Sheng H, Mitchell J, Warner T . Isoforms of nitric oxide synthase. Characterization and purification from different cell types. Biochem Pharmacol. 1991; 42(10):1849-57. DOI: 10.1016/0006-2952(91)90581-o. View

Mayer B, Schmidt K, Humbert P, Bohme E . Biosynthesis of endothelium-derived relaxing factor: a cytosolic enzyme in porcine aortic endothelial cells Ca2+-dependently converts L-arginine into an activator of soluble guanylyl cyclase. Biochem Biophys Res Commun. 1989; 164(2):678-85. DOI: 10.1016/0006-291x(89)91513-1. View

Narhi L, FULCO A . Characterization of a catalytically self-sufficient 119,000-dalton cytochrome P-450 monooxygenase induced by barbiturates in Bacillus megaterium. J Biol Chem. 1986; 261(16):7160-9. View

Berry E, Trumpower B . Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra. Anal Biochem. 1987; 161(1):1-15. DOI: 10.1016/0003-2697(87)90643-9. View

Omura T, Sato R . THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964; 239:2370-8. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Bredt D, Ferris C, Snyder S . Nitric oxide synthase regulatory sites. Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification of flavin and calmodulin binding sites. J Biol Chem. 1992; 267(16):10976-81. View

Boucher J, Genet A, Vadon S, Delaforge M, Mansuy D . Formation of nitrogen oxides and citrulline upon oxidation of N omega-hydroxy-L-arginine by hemeproteins. Biochem Biophys Res Commun. 1992; 184(3):1158-64. DOI: 10.1016/s0006-291x(05)80004-x. View

10.

Bredt D, Snyder S . Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc Natl Acad Sci U S A. 1990; 87(2):682-5. PMC: 53329. DOI: 10.1073/pnas.87.2.682. View

11.

Garthwaite J . Glutamate, nitric oxide and cell-cell signalling in the nervous system. Trends Neurosci. 1991; 14(2):60-7. DOI: 10.1016/0166-2236(91)90022-m. View

12.

Mayer B, John M, Heinzel B, Werner E, Wachter H, Schultz G . Brain nitric oxide synthase is a biopterin- and flavin-containing multi-functional oxido-reductase. FEBS Lett. 1991; 288(1-2):187-91. DOI: 10.1016/0014-5793(91)81031-3. View

13.

Moncada S, Palmer R, Higgs E . Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991; 43(2):109-42. View

14.

Ruettinger R, Wen L, FULCO A . Coding nucleotide, 5' regulatory, and deduced amino acid sequences of P-450BM-3, a single peptide cytochrome P-450:NADPH-P-450 reductase from Bacillus megaterium. J Biol Chem. 1989; 264(19):10987-95. View

15.

Haurand M, Ullrich V . Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme. J Biol Chem. 1985; 260(28):15059-67. View

16.

Gerzer R, Bohme E, Hofmann F, Schultz G . Soluble guanylate cyclase purified from bovine lung contains heme and copper. FEBS Lett. 1981; 132(1):71-4. DOI: 10.1016/0014-5793(81)80429-2. View

17.

Heinzel B, John M, Klatt P, Bohme E, Mayer B . Ca2+/calmodulin-dependent formation of hydrogen peroxide by brain nitric oxide synthase. Biochem J. 1992; 281 ( Pt 3):627-30. PMC: 1130735. DOI: 10.1042/bj2810627. View

18.

Klatt P, Heinzel B, John M, Kastner M, Bohme E, Mayer B . Ca2+/calmodulin-dependent cytochrome c reductase activity of brain nitric oxide synthase. J Biol Chem. 1992; 267(16):11374-8. View

19.

White K, Marletta M . Nitric oxide synthase is a cytochrome P-450 type hemoprotein. Biochemistry. 1992; 31(29):6627-31. DOI: 10.1021/bi00144a001. View

20.

Boucher J, Delaforge M, Henry Y, Mansuy D . Formation of nitric oxide by cytochrome P450-catalyzed oxidation of aromatic amidoximes. Biochem Biophys Res Commun. 1992; 185(1):452-8. DOI: 10.1016/s0006-291x(05)81006-x. View