Prediction of Accumulation of 131I-labelled Meta-iodobenzylguanidine in Neuroblastoma Cell Lines by Means of Reverse Transcription and Polymerase Chain Reaction

Overview

Journal Br J Cancer

Specialty Oncology

Date 1994 Jul 1

PMID 7517173

Citations 13

Authors

R J Mairs

A Livingstone

M N Gaze

T E Wheldon

A Barrett

Affiliations

Soon will be listed here.

Abstract

Radiolabelled meta-iodobenzylguanidine (mIBG) currently provides one of the most promising options for targeted radiotherapy of neuroblastoma. No means currently exists for prediction of mIBG uptake in tumour cells of individual patients other than semiquantitative inferences from diagnostic scanning which depend on the continued existence of a macroscopic tumour mass. A biological rapid assay which could be applied at initial biopsy would be invaluable in selecting patients for therapeutic strategies which incorporate radiolabelled mIBG. We have assessed the expression of the noradrenaline transporter gene in six human neuroblastoma cell lines and in three non-neural crest-derived cell lines using reverse transcription followed by the polymerase chain reaction. Transcription of this gene was observed in five out of six neuroblastoma cell lines but in none of the control cells. A highly significant correlation was established (P < 0.01) between gene expression and active cellular accumulation of mIBG. It is suggested that semiquantitative evaluation of noradrenaline transporter gene transcripts may be predictive of mIBG uptake by tumours in vivo.

Citing Articles

Immunohistochemical evaluation of molecular radiotherapy target expression in neuroblastoma tissue.

Gains J, Sebire N, Moroz V, Wheatley K, Gaze M Eur J Nucl Med Mol Imaging. 2017; 45(3):402-411.

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MIBG avidity correlates with clinical features, tumor biology, and outcomes in neuroblastoma: A report from the Children's Oncology Group.

Dubois S, Mody R, Naranjo A, Van Ryn C, Russ D, Oldridge D Pediatr Blood Cancer. 2017; 64(11).

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Vesicular monoamine transporter protein expression correlates with clinical features, tumor biology, and MIBG avidity in neuroblastoma: a report from the Children's Oncology Group.

Temple W, Mendelsohn L, Kim G, Nekritz E, Gustafson W, Lin L Eur J Nucl Med Mol Imaging. 2015; 43(3):474-481.

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Nothing but NET: a review of norepinephrine transporter expression and efficacy of 131I-mIBG therapy.

Streby K, Shah N, Ranalli M, Kunkler A, Cripe T Pediatr Blood Cancer. 2014; 62(1):5-11.

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High ¹²³I-MIBG uptake in neuroblastic tumours indicates unfavourable histopathology.

Fendler W, Melzer H, Walz C, von Schweinitz D, Coppenrath E, Schmid I Eur J Nucl Med Mol Imaging. 2013; 40(11):1701-10.

PMID: 23857458 DOI: 10.1007/s00259-013-2491-y.

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