Flow Cytometric Measurement of Immunoglobulin E to Natural Latex Proteins

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1994 Mar 1

PMID 7496945

Citations 1

Authors

P L Kwittken

N A Pawlowski

S K Sweinberg

S D Douglas

D E Campbell

Affiliations

Soon will be listed here.

Abstract

Immediate hypersensitivity to natural latex (NL) occurs in sensitized individuals after repeated exposure to products or devices containing NL components. Since allergic reactions to NL proteins are quite frequent and may be quite serious, diagnostic assays are needed to identify individuals at risk. A number of latex proteins have been considered the major antigens, but they have been incompletely characterized. There is no standard material available for skin testing. In vitro diagnostic tests, such as the radioallergosorbent test (RAST), are time consuming and their sensitivity and specificity remain to be proven. We have developed a rapid microsphere-based, fluorescence-activated flow cytometry assay for the measurement of NL protein-specific human immunoglobulin E and have compared it with both the enzyme-linked immunosorbent assay and radioallergosorbent test methods. By using the total purified NL protein fraction isolated from raw ammoniated NL sap as the antigen, the flow cytometry assay was both sensitive and specific for the detection of NL protein-specific human immunoglobulin E in the sera of sensitized pediatric patients.

Citing Articles

A comparison of electrochemiluminescence and flow cytometry for the detection of natural latex-specific human immunoglobulin E.

Kobrynski L, Tanimune L, Pawlowski N, Douglas S, Campbell D Clin Diagn Lab Immunol. 1996; 3(1):42-6.

PMID: 8770502 PMC: 170245. DOI: 10.1128/cdli.3.1.42-46.1996.

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